Chang H S, Yamato O, Sakai Y, Yamasaki M, Maede Y
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, 060-0818 Sapporo, Japan.
Prostaglandins Leukot Essent Fatty Acids. 2004 Jan;70(1):77-83. doi: 10.1016/j.plefa.2003.08.006.
We recently identified sodium n-propyl thiosulfate (NPTS) and sodium 2-propenyl thiosulfate (2PTS) from boiled onion and garlic, respectively, as causative agents of hemolytic anemia in dogs. We present here data concerning the effects of these alk(en)yl thiosulfates on superoxide (O(2)(-)) generation in peripheral polymorphonuclear leukocytes (PMNs) and on adenosine 5'-diphosphate (ADP)-induced platelet aggregation in dogs and humans in vitro. Both NPTS and 2PTS increased O(2)(-) generation significantly (P<0.05 at 1mM NPTS, P<0.005 at 0.1 and 1mM 2PTS) and reduced its reaction time significantly (P<0.05 between 0.01 and 1mM NPTS and at 1mM 2PTS) in canine PMNs stimulated by phorbol 12-myristate 13-acetate, compared with the control without alk(en)yl thiosulfates. However, a tendency to return to the control level was observed at 10mM of the alk(en)yl thiosulfates in both O(2)(-) generation and its reaction time. Although NPTS and 2PTS did not exert any significant effect on the O(2)(-) generation in human PMNs, 2PTS reduced its reaction time significantly (P<0.05) at 1 and 10mM compared with the control, showing that 2PTS accelerated O(2)(-) generation in human PMNs. The difference in effects on O(2)(-) generation may be due to that in susceptibility to alk(en)yl thiosulfates between canine and human PMNs. On the other hand, NPTS and 2PTS were shown to significantly inhibit ADP-induced platelet aggregation at 0.01mM (P<0.01) in canine platelets and at 0.001-0.1mM (P<0.05) in human platelets. In contrast, the maximal aggregation percentage returned to the control level at 1mM of alk(en)yl thiosulfates in both canine and human platelets. From these results, we conclude that NPTS and 2PTS have the potential to promote immune functions and prevent cardiovascular diseases.
我们最近分别从煮过的洋葱和大蒜中鉴定出正丙基硫代硫酸钠（NPTS）和2-丙烯基硫代硫酸钠（2PTS），它们是犬类溶血性贫血的致病因子。在此，我们展示了这些烷基（烯基）硫代硫酸盐对犬类和人类外周多形核白细胞（PMN）中超氧化物（O₂⁻）生成以及对二磷酸腺苷（ADP）诱导的血小板聚集的体外影响数据。与不含烷基（烯基）硫代硫酸盐的对照组相比，NPTS和2PTS均可显著增加犬类PMN中O₂⁻的生成（1mM NPTS时P<0.05，0.1mM和1mM 2PTS时P<0.005），并显著缩短其反应时间（0.01至1mM NPTS以及1mM 2PTS时P<0.05），这些是在用佛波酯12-肉豆蔻酸酯13-乙酸酯刺激的犬类PMN中观察到的。然而，在10mM烷基（烯基）硫代硫酸盐时，O₂⁻生成及其反应时间均出现了回到对照水平的趋势。尽管NPTS和2PTS对人类PMN中的O₂⁻生成没有显著影响，但与对照组相比，2PTS在1mM和10mM时可显著缩短其反应时间（P<0.05），表明2PTS可加速人类PMN中O₂⁻的生成。对O₂⁻生成影响的差异可能是由于犬类和人类PMN对烷基（烯基）硫代硫酸盐的敏感性不同。另一方面，NPTS和2PTS在犬类血小板中0.01mM时（P<0.01）以及人类血小板中0.001至0.1mM时（P<0.05）均显示出显著抑制ADP诱导的血小板聚集。相反，在1mM烷基（烯基）硫代硫酸盐时，犬类和人类血小板中的最大聚集百分比均回到对照水平。从这些结果中，我们得出结论，NPTS和2PTS具有促进免疫功能和预防心血管疾病的潜力。
