Laboratory of Clinical Pathology, Department of Veterinary Clinical Sciences, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan.
Nutr Res. 2010 Jun;30(6):435-40. doi: 10.1016/j.nutres.2010.06.007.
There is evidence that onions and garlic protect against cancer in humans. It has been suggested that this effect is partly due to the organosulfur compounds in Allium vegetables and that these substances act through induction of phase II detoxification enzymes. Here, we hypothesized that alk(en)yl thiosulfates, sodium n-propyl thiosulfate (NPTS), and sodium 2-propenyl thiosulfate (2PTS), which were identified in onions and garlic, respectively, may induce phase II enzymes. Therefore, rat hepatoma cells (H4IIE) were cultured with 1 to 100 micromol/L of NPTS or 2PTS for 48 hours at 37 degrees C; and the activities and messenger RNA (mRNA) expression levels of phase II enzymes in H4IIE cells were investigated. The effects of diallyl trisulfide and tert-butylhydroquinone, known as phase II inducers, were also examined as positive controls and compared with the responses of NPTS and 2PTS. Quinone reductase (QR) activity and mRNA expression levels of QR and epoxide hydrolase 1 were significantly increased by 2PTS (P < .05-.005). In particular, QR activity was increased at a relatively low concentration of 2PTS (10 micromol/L). However, glutathione S-transferase activity and mRNA expression levels of glutathione S-transferase A5 and uridine diphosphate glucuronosyl transferase 1A1 were not changed by 2PTS. In contrast, NPTS did not affect the activities and mRNA expression levels of these phase II enzymes. These results show that 2PTS can induce phase II enzymes, and its inductive effect is comparable or superior to that of diallyl trisulfide and tert-butylhydroquinone.
有证据表明洋葱和大蒜能预防人类癌症。有人提出，这种效果部分归因于大蒜属蔬菜中的有机硫化合物，而这些物质通过诱导 II 相解毒酶起作用。在这里，我们假设烯丙基硫代硫酸盐、正丙基硫代磺酸钠（NPTS）和烯丙基 2- 硫代磺酸钠（2PTS），分别在洋葱和大蒜中被鉴定出来，可能诱导 II 相酶。因此，将大鼠肝癌细胞（H4IIE）在 37°C 下用 1 至 100 微摩尔/升的 NPTS 或 2PTS 培养 48 小时；并研究 H4IIE 细胞中 II 相酶的活性和信使 RNA（mRNA）表达水平。还作为阳性对照研究了二烯丙基三硫醚和叔丁基对苯二酚，它们被认为是 II 相诱导剂，并与 NPTS 和 2PTS 的反应进行了比较。醌还原酶（QR）活性和 QR 和环氧化物水解酶 1 的 mRNA 表达水平均被 2PTS 显著增加（P<0.05-0.005）。特别是，QR 活性在相对较低的 2PTS 浓度（10 微摩尔/升）下增加。然而，谷胱甘肽 S-转移酶活性和谷胱甘肽 S-转移酶 A5 和尿苷二磷酸葡萄糖醛酸转移酶 1A1 的 mRNA 表达水平不受 2PTS 影响。相比之下，NPTS 不影响这些 II 相酶的活性和 mRNA 表达水平。这些结果表明，2PTS 可以诱导 II 相酶，其诱导作用可与二烯丙基三硫醚和叔丁基对苯二酚相媲美或优于后者。
