由替代西格玛因子AlgT控制铜绿假单胞菌algZ基因的表达
Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT.
作者信息
Wozniak Daniel J, Sprinkle April B, Baynham Patricia J
机构信息
Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA.
出版信息
J Bacteriol. 2003 Dec;185(24):7297-300. doi: 10.1128/JB.185.24.7297-7300.2003.
Abstract
AlgZ controls Pseudomonas aeruginosa alginate synthesis by activating algD, yet algZ expression is not detectable in nonmucoid strains. Mobility shift and Western blot assays revealed that algZ expression requires the sigma factor AlgT. The mapped algZ transcription start site revealed a consensus AlgT-dependent promoter that, when mutated, substantially reduced algZ transcription.
摘要
AlgZ通过激活algD来控制铜绿假单胞菌藻酸盐的合成，但在非黏液型菌株中检测不到AlgZ的表达。迁移率变动分析和蛋白质免疫印迹分析表明，AlgZ的表达需要σ因子AlgT。定位的algZ转录起始位点显示出一个依赖AlgT的共有启动子，该启动子发生突变时，会大幅降低algZ的转录水平。
相似文献
1
Control of Pseudomonas aeruginosa algZ expression by the alternative sigma factor AlgT.由替代西格玛因子AlgT控制铜绿假单胞菌algZ基因的表达
J Bacteriol. 2003 Dec;185(24):7297-300. doi: 10.1128/JB.185.24.7297-7300.2003.
2
Identification and characterization of AlgZ, an AlgT-dependent DNA-binding protein required for Pseudomonas aeruginosa algD transcription.铜绿假单胞菌藻酸盐合成基因algD转录所需的AlgT依赖性DNA结合蛋白AlgZ的鉴定与特性分析
Mol Microbiol. 1996 Oct;22(1):97-108. doi: 10.1111/j.1365-2958.1996.tb02659.x.
3
Pseudomonas aeruginosa AlgZ, a ribbon-helix-helix DNA-binding protein, is essential for alginate synthesis and algD transcriptional activation.铜绿假单胞菌AlgZ是一种带状-螺旋-螺旋DNA结合蛋白，对藻酸盐合成和algD转录激活至关重要。
Mol Microbiol. 1999 Sep;33(5):1069-80. doi: 10.1046/j.1365-2958.1999.01550.x.
4
Transcriptional analysis of the Pseudomonas aeruginosa genes algR, algB, and algD reveals a hierarchy of alginate gene expression which is modulated by algT.铜绿假单胞菌基因algR、algB和algD的转录分析揭示了由algT调节的藻酸盐基因表达层次结构。
J Bacteriol. 1994 Oct;176(19):6007-14. doi: 10.1128/jb.176.19.6007-6014.1994.
5
Mucoid-to-nonmucoid conversion in alginate-producing Pseudomonas aeruginosa often results from spontaneous mutations in algT, encoding a putative alternate sigma factor, and shows evidence for autoregulation.在产藻酸盐的铜绿假单胞菌中，黏液型到非黏液型的转变通常是由编码假定的替代西格玛因子的algT自发突变引起的，并显示出自调节的证据。
J Bacteriol. 1994 Nov;176(21):6677-87. doi: 10.1128/jb.176.21.6677-6687.1994.
6
The AlgT-dependent transcriptional regulator AmrZ (AlgZ) inhibits flagellum biosynthesis in mucoid, nonmotile Pseudomonas aeruginosa cystic fibrosis isolates.依赖AlgT的转录调节因子AmrZ（AlgZ）抑制黏液样、无运动性的铜绿假单胞菌囊性纤维化分离株中的鞭毛生物合成。
J Bacteriol. 2006 Sep;188(18):6483-9. doi: 10.1128/JB.00636-06.
7
Identification of the algZ gene upstream of the response regulator algR and its participation in control of alginate production in Pseudomonas aeruginosa.铜绿假单胞菌中应答调节因子algR上游algZ基因的鉴定及其在藻酸盐产生控制中的作用
J Bacteriol. 1997 Jan;179(1):187-93. doi: 10.1128/jb.179.1.187-193.1997.
8
Posttranslational control of the algT (algU)-encoded sigma22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN).铜绿假单胞菌中藻酸盐调节子表达的algT（algU）编码的σ22的翻译后调控及其拮抗蛋白MucA和MucB（AlgN）的定位
J Bacteriol. 1997 Jun;179(11):3711-20. doi: 10.1128/jb.179.11.3711-3720.1997.
9
Involvement of the alginate algT gene and integration host factor in the regulation of the Pseudomonas aeruginosa algB gene.藻酸盐algT基因和整合宿主因子参与铜绿假单胞菌algB基因的调控。
J Bacteriol. 1993 Jul;175(13):4145-53. doi: 10.1128/jb.175.13.4145-4153.1993.
10
The Pseudomonas aeruginosa ribbon-helix-helix DNA-binding protein AlgZ (AmrZ) controls twitching motility and biogenesis of type IV pili.铜绿假单胞菌的带状-螺旋-螺旋DNA结合蛋白AlgZ（AmrZ）控制IV型菌毛的颤动运动和生物合成。
J Bacteriol. 2006 Jan;188(1):132-40. doi: 10.1128/JB.188.1.132-140.2006.
引用本文的文献
1
The Azotobacter vinelandii AlgU regulon during vegetative growth and encysting conditions: A proteomic approach.威氏固氮菌 AlgU 调控组在营养生长和形成休眠包囊条件下的变化：一种蛋白质组学方法。
PLoS One. 2023 Nov 15;18(11):e0286440. doi: 10.1371/journal.pone.0286440. eCollection 2023.
2
Adaption of F113 to the Rhizosphere Environment-The AmrZ-FleQ Hub.F113对根际环境的适应性——AmrZ-FleQ枢纽
Microorganisms. 2023 Apr 15;11(4):1037. doi: 10.3390/microorganisms11041037.
3
The Two-Component System FleS/FleR Represses H1-T6SS via Cyclic di-GMP Signaling in Pseudomonas aeruginosa.铜绿假单胞菌中双组分系统 FleS/FleR 通过环二鸟苷酸信号抑制 H1-T6SS。
Appl Environ Microbiol. 2022 Jan 25;88(2):e0165521. doi: 10.1128/AEM.01655-21. Epub 2021 Nov 3.
4
The anti-sigma factor MucA is required for viability in Pseudomonas aeruginosa.MucA 抗西格玛因子是铜绿假单胞菌生存所必需的。
Mol Microbiol. 2021 Aug;116(2):550-563. doi: 10.1111/mmi.14732. Epub 2021 May 18.
5
Identification of Regulatory Genes and Metabolic Processes Important for Alginate Biosynthesis in by Screening of a Transposon Insertion Mutant Library.通过筛选转座子插入突变体文库鉴定对海藻酸盐生物合成重要的调控基因和代谢过程。 （注：原文中“in”后面缺少具体内容，这里按完整语义翻译）
Front Bioeng Biotechnol. 2020 Jan 17;7:475. doi: 10.3389/fbioe.2019.00475. eCollection 2019.
6
How bacteria recognise and respond to surface contact.细菌如何识别和响应表面接触。
FEMS Microbiol Rev. 2020 Jan 1;44(1):106-122. doi: 10.1093/femsre/fuz029.
7
Cystic Fibrosis and Pseudomonas aeruginosa: the Host-Microbe Interface.囊性纤维化与铜绿假单胞菌：宿主-微生物界面。
Clin Microbiol Rev. 2019 May 29;32(3). doi: 10.1128/CMR.00138-18. Print 2019 Jun 19.
8
Outer-membrane protein LptD (PA0595) plays a role in the regulation of alginate synthesis in Pseudomonas aeruginosa.外膜蛋白 LptD（PA0595）在铜绿假单胞菌中藻酸盐合成的调控中发挥作用。
J Med Microbiol. 2018 Aug;67(8):1139-1156. doi: 10.1099/jmm.0.000752. Epub 2018 Jun 20.
9
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in .RsmA 和 AmrZ 协调所有三种类型 VI 分泌系统在. 的组装。
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712. doi: 10.1073/pnas.1700286114. Epub 2017 Jul 3.
10
Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies.细菌胞外多糖：生物合成途径与工程策略
Front Microbiol. 2015 May 26;6:496. doi: 10.3389/fmicb.2015.00496. eCollection 2015.
本文引用的文献
1
Transcriptional analysis of genes involved in Pseudomonas aeruginosa biofilms.铜绿假单胞菌生物膜相关基因的转录分析
Methods Enzymol. 2001;336:144-51. doi: 10.1016/s0076-6879(01)36586-2.
2
Integration-proficient Pseudomonas aeruginosa vectors for isolation of single-copy chromosomal lacZ and lux gene fusions.用于分离单拷贝染色体lacZ和lux基因融合体的整合能力强的铜绿假单胞菌载体。
Biotechniques. 2000 Nov;29(5):948-50, 952. doi: 10.2144/00295bm04.
3
Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.铜绿假单胞菌PAO1（一种机会致病菌）的全基因组序列
Nature. 2000 Aug 31;406(6799):959-64. doi: 10.1038/35023079.
4
Regulation of high affinity nickel uptake in bacteria. Ni2+-Dependent interaction of NikR with wild-type and mutant operator sites.细菌中高亲和力镍摄取的调控。NikR 与野生型和突变型操纵子位点的 Ni2+ 依赖性相互作用。
J Biol Chem. 2000 Jun 30;275(26):19735-41. doi: 10.1074/jbc.M002232200.
5
Pseudomonas aeruginosa AlgZ, a ribbon-helix-helix DNA-binding protein, is essential for alginate synthesis and algD transcriptional activation.铜绿假单胞菌AlgZ是一种带状-螺旋-螺旋DNA结合蛋白，对藻酸盐合成和algD转录激活至关重要。
Mol Microbiol. 1999 Sep;33(5):1069-80. doi: 10.1046/j.1365-2958.1999.01550.x.
6
Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa.鉴定大肠杆菌pepA同源物及其在抑制黏液型铜绿假单胞菌algB表型中的作用。
J Bacteriol. 1999 Jan;181(1):107-16. doi: 10.1128/JB.181.1.107-116.1999.
7
A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants.一种用于特异性切除染色体定位DNA序列的广宿主范围Flp-FRT重组系统：在分离无标记铜绿假单胞菌突变体中的应用
Gene. 1998 May 28;212(1):77-86. doi: 10.1016/s0378-1119(98)00130-9.
8
Phosphorylation-independent activity of the response regulators AlgB and AlgR in promoting alginate biosynthesis in mucoid Pseudomonas aeruginosa.应答调节因子AlgB和AlgR在促进黏液型铜绿假单胞菌藻酸盐生物合成中的非磷酸化依赖性活性。
J Bacteriol. 1998 Feb;180(4):956-68. doi: 10.1128/JB.180.4.956-968.1998.
9
Identification and characterization of AlgZ, an AlgT-dependent DNA-binding protein required for Pseudomonas aeruginosa algD transcription.铜绿假单胞菌藻酸盐合成基因algD转录所需的AlgT依赖性DNA结合蛋白AlgZ的鉴定与特性分析
Mol Microbiol. 1996 Oct;22(1):97-108. doi: 10.1111/j.1365-2958.1996.tb02659.x.
10
Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia.囊性纤维化中的微生物致病机制：黏液型铜绿假单胞菌和洋葱伯克霍尔德菌
Microbiol Rev. 1996 Sep;60(3):539-74. doi: 10.1128/mr.60.3.539-574.1996.
Zotero 插件