Bryan B A, McGrew E, Lu Y, Polymenis M
Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843, USA.
Mol Genet Genomics. 2004 Feb;271(1):72-81. doi: 10.1007/s00438-003-0957-5. Epub 2003 Nov 27.
It is generally thought that cell growth and metabolism regulate cell division and not vice versa. Here, we examined Saccharomyces cerevisiae cells growing under conditions of continuous culture in a chemostat. We found that loss of G1 cyclins, or inactivation of the cyclin-dependent kinase Cdc28p, reduced the activity of glutamate synthase (Glt1p), a key enzyme in nitrogen assimilation. We also present evidence indicating that the G1 cyclin-dependent control of Glt1p may involve Jem1p, a DnaJ-type chaperone. Our results suggest that completion of START may be linked to nitrogen metabolism.
一般认为，细胞生长和代谢调节细胞分裂，反之则不然。在这里，我们研究了在恒化器中连续培养条件下生长的酿酒酵母细胞。我们发现，G1 细胞周期蛋白的缺失或细胞周期蛋白依赖性激酶 Cdc28p 的失活会降低谷氨酸合酶（Glt1p）的活性，谷氨酸合酶是氮同化中的关键酶。我们还提供了证据表明，Glt1p 的 G1 细胞周期蛋白依赖性控制可能涉及 Jem1p，一种 DnaJ 型伴侣蛋白。我们的结果表明，START 的完成可能与氮代谢有关。
