Wu Chin-Lee, Taylor Jennifer L, He Wenlei, Zepeda Andrea G, Halpern Elkan F, Bielecki Anthony, Gonzalez R Gilberto, Cheng Leo L
Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Magn Reson Med. 2003 Dec;50(6):1307-11. doi: 10.1002/mrm.10645.
The previously observed improvement in spectral resolution of tissue proton NMR with high-resolution magic angle spinning (HRMAS) was speculated to be due largely to freeze-thawing artifacts resulting from tissue storage. In this study, 12 human prostate samples were analyzed on a 14.1T spectrometer at 3 degrees C, with HRMAS rates of 600 and 700 Hz. These samples were measured fresh and after they were frozen for 12-16 hr prior to thawing. The spectral linewidths measured from fresh and previously frozen samples were identical for all metabolites except citrate and acetate. The metabolite intensities of fresh and freeze-thawed samples depend on the quantification procedures used; however, in this experiment the differences of means were <30%. As expected, it was found that tissue storage impacts tissue quality for pathological analysis, and HRMAS conditions alone are not sufficiently destructive to impair pathological evaluation. Furthermore, although storage conditions affect absolute metabolite concentrations in NMR analysis, relative metabolite concentrations are less affected.
先前观察到的采用高分辨率魔角旋转（HRMAS）技术提高组织质子核磁共振光谱分辨率的现象，据推测很大程度上是由于组织储存过程中产生的冻融假象。在本研究中，12个人类前列腺样本在3摄氏度的14.1T光谱仪上进行分析，HRMAS速率为600和700Hz。这些样本在新鲜时以及在解冻前冷冻12 - 16小时后进行测量。除柠檬酸盐和乙酸盐外，新鲜样本和先前冷冻样本测量得到的所有代谢物的谱线宽度均相同。新鲜样本和冻融样本的代谢物强度取决于所使用的定量程序；然而，在本实验中，平均值差异小于30%。正如预期的那样，发现组织储存会影响用于病理分析的组织质量，并且仅HRMAS条件不足以破坏到损害病理评估。此外，尽管储存条件会影响核磁共振分析中代谢物的绝对浓度，但相对代谢物浓度受影响较小。
