Departament de Bioquímica i Biologia Molecular, Unitat de Bioquímica de Biociències, Edifici Cs, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Valle`s, Spain.
MAGMA. 2012 Oct;25(5):401-10. doi: 10.1007/s10334-012-0303-1.
High-resolution magic angle spinning (HRMAS) magnetic resonance spectroscopy provides detailed metabolomic information from intact tissue. However, long acquisition times and high rotation speed may lead to timedependent spectral pattern changes, which may affect proper interpretation of results. We report a strategy to minimize those changes, even at physiological recording temperature.
Glioblastoma(Gbm) tumours were induced in 12 mice by stereotactic injection of GL261 cells. Animals were sacrificed and tumours were removed and stored in liquid N2. Half of the samples were exposed to focused microwave (FMW) irradiation prior to HRMAS while the other half was not. Time-course experiments (374 min at 37°C, 9.4T, 3,000 Hz spinning rate) were carried out to monitor spectral pattern changes. Differences were assessed with Unianova test while post-HRMAS histopathology analysis was performed to assess tissue integrity.
Significant changes (up to 1.7 fold) were observed in samples without FMW irradiation in several spectral regions e.g. mobile lipids/lactate (0.90-1.30 ppm), acetate (1.90 ppm), N-acetyl aspartate (2.00 ppm), and Choline-containing compounds (3.19-3.25 ppm). No significant changes in the spectral pattern of FMW-irradiated samples were recorded.
We describe here a successful strategy to minimize spectral pattern changes in mouse Gbm samples using a FMW irradiation system.
高分辨率魔角旋转（HRMAS）磁共振波谱提供了来自完整组织的详细代谢组学信息。然而，较长的采集时间和较高的旋转速度可能导致随时间变化的光谱模式变化，这可能会影响对结果的正确解释。我们报告了一种策略，可以最小化这些变化，即使在生理记录温度下也是如此。
通过立体定向注射 GL261 细胞在 12 只小鼠中诱导脑胶质瘤（GBM）肿瘤。动物被处死，肿瘤被取出并储存在液氮中。一半的样本在进行 HRMAS 之前暴露于聚焦微波（FMW）辐射下，而另一半则没有。进行了时间过程实验（在 37°C、9.4T、3000Hz 旋转速度下进行 374 分钟）以监测光谱模式变化。使用 Unianova 检验评估差异，同时进行 HRMAS 后组织病理学分析以评估组织完整性。
在没有 FMW 辐射的样本中，在几个光谱区域观察到显著变化（高达 1.7 倍），例如移动脂质/乳酸（0.90-1.30ppm）、乙酸盐（1.90ppm）、N-乙酰天冬氨酸（2.00ppm）和胆碱化合物（3.19-3.25ppm）。未记录到 FMW 辐照样本光谱模式的显著变化。
我们在这里描述了一种使用 FMW 辐照系统成功最小化小鼠 GBM 样本光谱模式变化的策略。
