Krajewski W A, Razin S V
Institute of Developmental Biology, Russian Academy of Sciences, Moscow.
Mol Gen Genet. 1992 Nov;235(2-3):381-8. doi: 10.1007/BF00279384.
The distribution of specific DNA sequence elements in a 2.9 kb HindIII fragment of chicken DNA containing the replication origin and the upstream matrix attachment site (MAR) of the alpha-globin gene domain was investigated. The fragment was shown to contain a CR1-type repetitive element and two stably bent DNA sequences. One of them colocalizes with the previously described MAR element and with the recognition site for a proliferating-cell-specific, DNA-binding protein. The melting pattern of a set of subfragments of the region proved to be non random. No correlation between the distribution of readily melting sequences and bent DNA was found. The possible importance of curved, low-melting and repetitive DNA sequences for the organization of the upstream boundary of the alpha-globin gene domain and the function of the replication origin is discussed.
研究了鸡DNA 2.9 kb HindIII片段中特定DNA序列元件的分布情况，该片段包含α-珠蛋白基因结构域的复制起点和上游基质附着位点（MAR）。结果表明，该片段含有一个CR1型重复元件和两个稳定弯曲的DNA序列。其中一个与先前描述的MAR元件以及增殖细胞特异性DNA结合蛋白的识别位点共定位。该区域一组亚片段的解链模式被证明是非随机的。未发现易解链序列的分布与弯曲DNA之间存在相关性。文中讨论了弯曲、低解链温度和重复DNA序列对于α-珠蛋白基因结构域上游边界组织以及复制起点功能的可能重要性。
