de Moura Gallo C V, Vassetzky Y S, Targa F R, Georgiev G P, Scherrer K, Razin S V
Institut Jacques Monod, Université Paris, France.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):512-9. doi: 10.1016/0006-291x(91)91401-w.
Several recognition sites for novel sequence-specific DNA-binding proteins were found at the 5'-side of the chicken alpha-globin gene domain in a 1.7 Kbp DNA fragment. This fragment includes the replication origin, a non tissue-specific transcriptional enhancer, a DNAse I hypersensitive site and a permanent site of DNA attachment to the nuclear matrix. Most of the identified protein binding sites differ from previously known consensus sequences. Two sites coincide with MARs located at the 5'-end of the 1.7 kbp fragment. The proteins interacting with these two recognition sites were observed only in proliferating cells and were virtually absent in the extracts obtained from the nonreplicating differentiated form of the same cells. One of them seems to belong to the GATA protein family, but its presence in nuclear extracts correlates with cell proliferation rather than expression of the domain.
在一个1.7千碱基对（kbp）的DNA片段中，鸡α-珠蛋白基因结构域的5'端发现了几个新型序列特异性DNA结合蛋白的识别位点。该片段包括复制起点、一个非组织特异性转录增强子、一个DNA酶I超敏位点以及DNA与核基质附着的永久位点。大多数已鉴定的蛋白质结合位点与先前已知的共有序列不同。两个位点与位于1.7 kbp片段5'端的基质附着区域（MARs）重合。仅在增殖细胞中观察到与这两个识别位点相互作用的蛋白质，而在从相同细胞的非复制分化形式获得的提取物中几乎不存在。其中一个似乎属于GATA蛋白家族，但其在核提取物中的存在与细胞增殖相关，而非该结构域的表达。
