Asrari Mahshid, Lobner Doug
Department of Endodontics, Marquette University School of Dentistry, Milwaukee, WI 53233, USA.
J Endod. 2003 Nov;29(11):743-6. doi: 10.1097/00004770-200311000-00014.
Root-end-filling materials have been tested for toxicity on several cell types, but their toxicity has not been tested on neurons. In this study we evaluated the neurotoxicity in murine cerebral cortical cell cultures of four commonly used root-end-filling materials: mineral trioxide aggregate, amalgam, Super EBA, and Diaket. Standardized amounts of each material were placed on culture-well inserts, allowing the material to be exposed to the culture bathing media without causing physical disruption of the cells. Cell death was quantified by assaying release of the cytosolic enzyme lactate dehydrogenase. Exposure of cortical cultures to freshly mixed or 7-day-old MTA did not cause significant neuronal death, whereas exposure to freshly mixed or 7-day-old amalgam, Super EBA, and Diaket resulted in significant neuronal death (p < .05). Thus, each material, except for mineral trioxide aggregate, can induce neurotoxicity, even when allowed to set thoroughly.
根尖充填材料已在多种细胞类型上进行了毒性测试，但尚未在神经元上测试其毒性。在本研究中，我们评估了四种常用根尖充填材料（三氧化矿物凝聚体、汞合金、Super EBA和Diaket）对小鼠大脑皮质细胞培养物的神经毒性。将每种材料的标准化量置于培养孔插入物上，使材料暴露于培养浴液中而不会对细胞造成物理破坏。通过检测胞质酶乳酸脱氢酶的释放来定量细胞死亡。将皮质培养物暴露于新鲜混合或7日龄的MTA不会导致显著的神经元死亡，而暴露于新鲜混合或7日龄的汞合金、Super EBA和Diaket则会导致显著的神经元死亡（p < 0.05）。因此，除三氧化矿物凝聚体外，每种材料即使在充分凝固后也可诱导神经毒性。
