使用PC12细胞评估聚己内酯和胶原微载体在神经导管制造中的应用。

Using PC12 cells to evaluate poly(caprolactone) and collagenous microcarriers for applications in nerve guide fabrication.

作者信息

Waddell Rebecca L, Marra Kacey G, Collins Kelly L, Leung Jade T, Doctor John S

机构信息

Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282, USA.

出版信息

Biotechnol Prog. 2003 Nov-Dec;19(6):1767-74. doi: 10.1021/bp034086m.

DOI:10.1021/bp034086m

PMID:14656154

Abstract

Tissue-engineered nerve guides can provide mechanical support as well as chemical stimulation for nerve regeneration. PC12 cells were used to test the novel combination of poly(caprolactone) (PCL) and macroporous collagen-based microcarriers (CultiSphers) as an initial phase in the fabrication of multichanneled nerve guides. Laminin-coated PCL was an effective matrix for the attachment, proliferation, and viability of PC12 cells, relative to uncoated PCL. PC12 cells attached to laminin-coated PCL and extended neurites when cultured in the presence of nerve growth factor (NGF). PC12 cells attached and proliferated on CultiSphers and extended neurites in response to NGF. A novel PCL/CultiSpher composite material also supported PC12 attachment and proliferation and provides a potentially useful material for the fabrication of synthetic nerve guides.

摘要

组织工程神经导管可为神经再生提供机械支持以及化学刺激。在制造多通道神经导管的初始阶段，使用PC12细胞来测试聚己内酯（PCL）与基于大孔胶原蛋白的微载体（CultiSphers）的新型组合。相对于未涂层的PCL，层粘连蛋白涂层的PCL是PCk细胞附着、增殖和存活的有效基质。当在神经生长因子（NGF）存在下培养时，PCk细胞附着于层粘连蛋白涂层的PCL并伸出神经突。PCk细胞在CultiSphers上附着并增殖，并对NGF作出反应伸出神经突。一种新型的PCL/CultiSphers复合材料也支持PCk细胞的附着和增殖，并为合成神经导管的制造提供了一种潜在有用的材料。