Devey M E, Carson S D, Nolan M F, Matheson A C, Te Riini C, Hohepa J
CSIRO Forestry and Forest Products, ACT 2604, Canberra, Australia.
Theor Appl Genet. 2004 Feb;108(3):516-24. doi: 10.1007/s00122-003-1446-2. Epub 2003 Dec 5.
A large full-sib family of radiata pine ( Pinus radiata Donn. ex D. Don) was used for quantitative trait locus (QTL) detection and independent verification. QTL detection experiments were carried out for juvenile wood density (JWD) and stem diameter at breast height (DBH) using selective genotyping. Evenly spaced RFLP and microsatellite markers were selected from an existing linkage map. QTLs were verified in an independent set of progeny from the same family. Based on map location, at least eight QTL positions for JWD and two for DBH were detected and verified. The percent variance accounted for by the markers ranged from 0.78% to 3.58%, suggesting a genomic architecture of many genes with small effect. Two unrelated "bridging" families were chosen as candidates for marker-aided selection (MAS), and six microsatellite markers showing an association with JWD or DBH were tested in these families. Of these, four markers showed a consistent association with JWD in one or both of the bridging families. Results from this study provide a basis for MAS in P. radiata.
一个辐射松（Pinus radiata Donn. ex D. Don）的大型全同胞家系被用于数量性状位点（QTL）检测和独立验证。使用选择性基因分型对幼龄材密度（JWD）和胸径（DBH）进行了QTL检测实验。从现有的连锁图谱中选择了均匀分布的RFLP和微卫星标记。在来自同一家系的独立子代群体中对QTL进行了验证。基于图谱位置，检测并验证了至少8个JWD的QTL位置和2个DBH的QTL位置。标记所解释的变异百分比范围为0.78%至3.58%，表明基因组结构由许多效应较小的基因组成。选择了两个不相关的“桥梁”家系作为标记辅助选择（MAS）的候选家系，并在这些家系中测试了6个与JWD或DBH相关的微卫星标记。其中，4个标记在一个或两个桥梁家系中与JWD表现出一致的相关性。本研究结果为辐射松的MAS提供了依据。
