Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, 311400, China.
Zhejiang Provincial Key Laboratory of Tree Breeding, Hangzhou, 311400, China.
BMC Plant Biol. 2019 Jan 25;19(1):41. doi: 10.1186/s12870-019-1655-8.
Schima superba (Theaceae) is a popular woody tree in China. The obscure chromosomal characters of this species are a limitation in the development of high-density genetic linkage maps, which are valuable resources for molecular breeding and functional genomics.
We determined the chromosome number and the karyotype of S. superba as 2n = 36 = 36 m, which is consistent with the tribe Schimeae (n = 18). A high-density genetic map was constructed using genotyping by sequencing (GBS). A F1 full-sib with 116 individuals and their parents (LC31 × JO32) were sequenced on the Illumina HiSeq™ platform. Overall, 343.3 Gb of raw data containing 1,191,933,474 paired-end reads were generated. Based on this, 99,966 polymorphic SNP markers were developed from the parents, and 2209 markers were mapped onto the integrated genetic linkage map after data filtering and SNP genotyping. The map spanned 2076.24 cM and was distributed among 18 linkage groups. The average marker interval was 0.94 cM. A total of 168 quantitative trait loci (QTLs) for 14 growth traits were identified.
The chromosome number and karyotype of S. superba was 2n = 36 = 36 m and a linkage map with 2209 SNP markers was constructed to identify QTLs for growth traits. Our study provides a basis for molecular-assisted breeding and genomic studies, which will contribute towards the future research and genetic improvement of S. superba.
木荷（山茶科）是中国一种受欢迎的木本树种。该物种染色体特征不明显，这限制了高密度遗传图谱的发展，而高密度遗传图谱是分子育种和功能基因组学的宝贵资源。
我们确定了木荷的染色体数目和核型为 2n = 36 = 36m，与该族（n = 18）一致。使用测序基因型（GBS）构建了高密度遗传图谱。对 116 个个体及其父母（LC31×JO32）的 F1 全同胞进行了 Illumina HiSeq™平台测序。总的来说，产生了 343.3Gb 的原始数据，包含 1191933474 对末端读取。在此基础上，从父母中开发了 99966 个多态性 SNP 标记，并且在数据过滤和 SNP 分型后，将 2209 个标记映射到综合遗传连锁图谱上。图谱跨越 2076.24cM，分布在 18 个连锁群中。标记的平均间隔为 0.94cM。鉴定出 14 个生长性状的 168 个数量性状位点（QTL）。
木荷的染色体数目和核型为 2n = 36 = 36m，构建了包含 2209 个 SNP 标记的连锁图谱，用于鉴定生长性状的 QTL。我们的研究为分子辅助育种和基因组学研究提供了基础，这将有助于未来对木荷的研究和遗传改良。
