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Evaluation of enzyme-linked immunosorbent assays and a PCR test for detection of shiga toxins for shiga toxin-producing Escherichia coli in cattle herds.用于检测牛群中产志贺毒素大肠杆菌志贺毒素的酶联免疫吸附测定和聚合酶链反应检测方法的评估。
J Clin Microbiol. 2003 Dec;41(12):5760-3. doi: 10.1128/JCM.41.12.5760-5763.2003.
2
Shiga Toxin Subtypes of Non-O157 Serogroups Isolated from Cattle Feces.从牛粪中分离出的非O157血清群的志贺毒素亚型
Front Cell Infect Microbiol. 2017 Apr 11;7:121. doi: 10.3389/fcimb.2017.00121. eCollection 2017.
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Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef.从海鲜和牛肉中分离出的产志贺毒素大肠杆菌（STEC）的特性分析。
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本文引用的文献

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A long-term study on the prevalence of shiga toxin-producing Escherichia coli (STEC) on four German cattle farms.一项关于德国四个养牛场中产志贺毒素大肠杆菌（STEC）流行情况的长期研究。
Epidemiol Infect. 2002 Aug;129(1):173-85. doi: 10.1017/s0950268802007288.
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[Verotoxin-producing E. coli (VTEC) in feces from cattle slaughtered in Germany].[德国屠宰牛粪便中产志贺毒素大肠杆菌（VTEC）]
Berl Munch Tierarztl Wochenschr. 1997 Apr;110(4):121-7.
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Mitomycin immunoblot colony assay for detection of Shiga-like toxin-producing Escherichia coli in fecal samples: comparison with DNA probes.用于检测粪便样本中产生志贺样毒素大肠杆菌的丝裂霉素免疫印迹菌落测定法：与DNA探针的比较
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Application of monoclonal antibody-based sandwich ELISAs to detect verotoxins in cattle faeces.
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A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests.一种用于选择临界值和定义血清学诊断试验中间结果的改良ROC分析。
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Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR.通过错配扩增突变分析-多重聚合酶链反应同时鉴定大肠杆菌O157:H7血清型菌株及其志贺样毒素类型。
J Clin Microbiol. 1995 Jan;33(1):248-50. doi: 10.1128/jcm.33.1.248-250.1995.
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Quantitative microtiter cytotoxicity assay for Shigella toxin.志贺毒素的定量微量滴定细胞毒性测定
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Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification.利用聚合酶链反应DNA扩增技术检测新生儿尿液中的巨细胞病毒。
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Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.用于通过聚合酶链反应扩增不同志贺样毒素基因片段的单一引物对。
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用于检测牛群中产志贺毒素大肠杆菌志贺毒素的酶联免疫吸附测定和聚合酶链反应检测方法的评估。

Evaluation of enzyme-linked immunosorbent assays and a PCR test for detection of shiga toxins for shiga toxin-producing Escherichia coli in cattle herds.

作者信息

Segura-Alvarez Monserrat, Richter Heinz, Conraths Franz J, Geue Lutz

机构信息

Federal Research Centre for Virus Diseases of Animals, Institute of Epidemiology, D-16868 Wusterhausen. Bundesinstitut für Risikobewertung, D-06846 Dessau, Germany.

出版信息

J Clin Microbiol. 2003 Dec;41(12):5760-3. doi: 10.1128/JCM.41.12.5760-5763.2003.

DOI:10.1128/JCM.41.12.5760-5763.2003
PMID:14662977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308972/
Abstract

Antigen capture enzyme-linked immunosorbent assays (ELISAs) for the detection of Stx1 and/or Stx2 in cattle feces were validated in comparison to the Vero cell cytotoxicity neutralization test (as a "gold standard") applied in the course of a monitoring program for Shiga toxin-producing Escherichia coli in German cattle herds as a prescreening test and compared to MK1/MK2 PCR as an alternative prescreening test.

摘要

在德国牛群产志贺毒素大肠杆菌监测项目中，为检测牛粪中的Stx1和/或Stx2，抗原捕获酶联免疫吸附测定（ELISA）作为一种预筛检测方法，与作为“金标准”的Vero细胞细胞毒性中和试验进行了验证比较，并与作为另一种预筛检测方法的MK1/MK2聚合酶链反应（PCR）进行了比较。