用于检测牛群中产志贺毒素大肠杆菌志贺毒素的酶联免疫吸附测定和聚合酶链反应检测方法的评估。
Evaluation of enzyme-linked immunosorbent assays and a PCR test for detection of shiga toxins for shiga toxin-producing Escherichia coli in cattle herds.
作者信息
Segura-Alvarez Monserrat, Richter Heinz, Conraths Franz J, Geue Lutz
机构信息
Federal Research Centre for Virus Diseases of Animals, Institute of Epidemiology, D-16868 Wusterhausen. Bundesinstitut für Risikobewertung, D-06846 Dessau, Germany.
出版信息
J Clin Microbiol. 2003 Dec;41(12):5760-3. doi: 10.1128/JCM.41.12.5760-5763.2003.
Abstract
Antigen capture enzyme-linked immunosorbent assays (ELISAs) for the detection of Stx1 and/or Stx2 in cattle feces were validated in comparison to the Vero cell cytotoxicity neutralization test (as a "gold standard") applied in the course of a monitoring program for Shiga toxin-producing Escherichia coli in German cattle herds as a prescreening test and compared to MK1/MK2 PCR as an alternative prescreening test.
摘要
在德国牛群产志贺毒素大肠杆菌监测项目中，为检测牛粪中的Stx1和/或Stx2，抗原捕获酶联免疫吸附测定（ELISA）作为一种预筛检测方法，与作为“金标准”的Vero细胞细胞毒性中和试验进行了验证比较，并与作为另一种预筛检测方法的MK1/MK2聚合酶链反应（PCR）进行了比较。
相似文献
1
Evaluation of enzyme-linked immunosorbent assays and a PCR test for detection of shiga toxins for shiga toxin-producing Escherichia coli in cattle herds.用于检测牛群中产志贺毒素大肠杆菌志贺毒素的酶联免疫吸附测定和聚合酶链反应检测方法的评估。
J Clin Microbiol. 2003 Dec;41(12):5760-3. doi: 10.1128/JCM.41.12.5760-5763.2003.
2
Shiga Toxin Subtypes of Non-O157 Serogroups Isolated from Cattle Feces.从牛粪中分离出的非O157血清群的志贺毒素亚型
Front Cell Infect Microbiol. 2017 Apr 11;7:121. doi: 10.3389/fcimb.2017.00121. eCollection 2017.
3
Analysis of the clonal relationship of shiga toxin-producing Escherichia coli serogroup O165:H25 isolated from cattle.对从牛身上分离出的产志贺毒素大肠杆菌血清型O165:H25的克隆关系分析。
Appl Environ Microbiol. 2006 Mar;72(3):2254-9. doi: 10.1128/AEM.72.3.2254-2259.2006.
4
Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef.从海鲜和牛肉中分离出的产志贺毒素大肠杆菌（STEC）的特性分析。
FEMS Microbiol Lett. 2004 Apr 1;233(1):173-8. doi: 10.1016/j.femsle.2004.02.008.
5
Comparative evaluation of the Ridascreen Verotoxin enzyme immunoassay for detection of Shiga-toxin producing strains of Escherichia coli (STEC) from food and other sources.用于检测食品及其他来源中产志贺毒素大肠杆菌（STEC）的Ridascreen Verotoxin酶免疫测定法的比较评估。
J Appl Microbiol. 2007 Mar;102(3):630-9. doi: 10.1111/j.1365-2672.2006.03139.x.
6
7
Shiga-toxin genes and genetic diversity of Escherichia coli isolated from pasteurized cow milk in Brazil.巴西巴氏杀菌牛奶中分离的大肠杆菌志贺毒素基因及遗传多样性
J Food Sci. 2014 Jun;79(6):M1175-80. doi: 10.1111/1750-3841.12477.
8
Probiotic Escherichia coli Nissle 1917 reduces growth, Shiga toxin expression, release and thus cytotoxicity of enterohemorrhagic Escherichia coli.益生菌大肠杆菌Nissle 1917可降低肠出血性大肠杆菌的生长、志贺毒素表达、释放，从而降低其细胞毒性。
Int J Med Microbiol. 2015 Jan;305(1):20-6. doi: 10.1016/j.ijmm.2014.10.003. Epub 2014 Oct 27.
9
Detection and characterization of Shiga toxin-producing Escherichia coli from seagulls.海鸥中产志贺毒素大肠杆菌的检测与鉴定
Epidemiol Infect. 2000 Aug;125(1):55-61. doi: 10.1017/s0950268899004100.
10
Multiple antibiotic resistances among Shiga toxin producing Escherichia coli O157 in feces of dairy cattle farms in Eastern Cape of South Africa.南非东开普省奶牛场粪便中产志贺毒素大肠杆菌O157的多重抗生素耐药性
BMC Microbiol. 2015 Oct 16;15:213. doi: 10.1186/s12866-015-0553-y.
引用本文的文献
1
Shiga Toxin-Producing E. coli in Animals: Detection, Characterization, and Virulence Assessment.动物中产志贺毒素大肠杆菌：检测、特性描述及毒力评估。
Methods Mol Biol. 2021;2291:19-86. doi: 10.1007/978-1-0716-1339-9_2.
2
Rapid microarray-based genotyping of enterohemorrhagic Escherichia coli serotype O156:H25/H-/Hnt isolates from cattle and clonal relationship analysis.基于快速微阵列的牛源产志贺样毒素大肠杆菌 O156:H25/H-/Hnt 血清型的基因分型及克隆关系分析。
Appl Environ Microbiol. 2010 Aug;76(16):5510-9. doi: 10.1128/AEM.00743-10. Epub 2010 Jun 25.
3
Analysis of the clonal relationship of serotype O26:H11 enterohemorrhagic Escherichia coli isolates from cattle.分析来自牛的血清型 O26:H11 肠出血性大肠杆菌分离株的克隆关系。
Appl Environ Microbiol. 2009 Nov;75(21):6947-53. doi: 10.1128/AEM.00605-09. Epub 2009 Sep 4.
4
Analysis of the clonal relationship of shiga toxin-producing Escherichia coli serogroup O165:H25 isolated from cattle.对从牛身上分离出的产志贺毒素大肠杆菌血清型O165:H25的克隆关系分析。
Appl Environ Microbiol. 2006 Mar;72(3):2254-9. doi: 10.1128/AEM.72.3.2254-2259.2006.
5
Transcriptional analysis of genes encoding Shiga toxin 2 and its variants in Escherichia coli.大肠杆菌中编码志贺毒素2及其变体的基因的转录分析。
Appl Environ Microbiol. 2005 Jan;71(1):558-61. doi: 10.1128/AEM.71.1.558-561.2005.
6
Prevalence and characterization of shiga toxin-producing Escherichia coli in swine feces recovered in the National Animal Health Monitoring System's Swine 2000 study.在国家动物卫生监测系统的2000年猪研究中采集的猪粪便中产志贺毒素大肠杆菌的流行情况及特征
Appl Environ Microbiol. 2004 Dec;70(12):7173-8. doi: 10.1128/AEM.70.12.7173-7178.2004.
本文引用的文献
1
A long-term study on the prevalence of shiga toxin-producing Escherichia coli (STEC) on four German cattle farms.一项关于德国四个养牛场中产志贺毒素大肠杆菌（STEC）流行情况的长期研究。
Epidemiol Infect. 2002 Aug;129(1):173-85. doi: 10.1017/s0950268802007288.
2
[Verotoxin-producing E. coli (VTEC) in feces from cattle slaughtered in Germany].[德国屠宰牛粪便中产志贺毒素大肠杆菌（VTEC）]
Berl Munch Tierarztl Wochenschr. 1997 Apr;110(4):121-7.
3
Studies on the development and use of a monoclonal sandwich ELISA for the detection of verotoxic Escherichia coli in animal faeces.
Vet Rec. 1997 Feb 1;140(5):112-5. doi: 10.1136/vr.140.5.112.
4
Mitomycin immunoblot colony assay for detection of Shiga-like toxin-producing Escherichia coli in fecal samples: comparison with DNA probes.用于检测粪便样本中产生志贺样毒素大肠杆菌的丝裂霉素免疫印迹菌落测定法：与DNA探针的比较
J Clin Microbiol. 1993 May;31(5):1167-72. doi: 10.1128/jcm.31.5.1167-1172.1993.
5
Application of monoclonal antibody-based sandwich ELISAs to detect verotoxins in cattle faeces.
Res Vet Sci. 1994 Sep;57(2):225-32. doi: 10.1016/0034-5288(94)90062-0.
6
A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests.一种用于选择临界值和定义血清学诊断试验中间结果的改良ROC分析。
J Immunol Methods. 1995 Sep 11;185(1):123-32. doi: 10.1016/0022-1759(95)00121-p.
7
Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR.通过错配扩增突变分析-多重聚合酶链反应同时鉴定大肠杆菌O157:H7血清型菌株及其志贺样毒素类型。
J Clin Microbiol. 1995 Jan;33(1):248-50. doi: 10.1128/jcm.33.1.248-250.1995.
8
Quantitative microtiter cytotoxicity assay for Shigella toxin.志贺毒素的定量微量滴定细胞毒性测定
J Clin Microbiol. 1980 Sep;12(3):361-6. doi: 10.1128/jcm.12.3.361-366.1980.
9
Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification.利用聚合酶链反应DNA扩增技术检测新生儿尿液中的巨细胞病毒。
J Infect Dis. 1988 Dec;158(6):1177-84. doi: 10.1093/infdis/158.6.1177.
10
Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.用于通过聚合酶链反应扩增不同志贺样毒素基因片段的单一引物对。
J Clin Microbiol. 1989 Dec;27(12):2751-7. doi: 10.1128/jcm.27.12.2751-2757.1989.
Zotero 插件