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Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR.通过错配扩增突变分析-多重聚合酶链反应同时鉴定大肠杆菌O157:H7血清型菌株及其志贺样毒素类型。
J Clin Microbiol. 1995 Jan;33(1):248-50. doi: 10.1128/jcm.33.1.248-250.1995.
2
Detection of Escherichia coli O157:H7 by multiplex PCR.通过多重聚合酶链反应检测大肠杆菌O157:H7
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3
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A simple filtration technique to detect enterohemorrhagic Escherichia coli O157:H7 and its toxins in beef by multiplex PCR.一种通过多重聚合酶链反应检测牛肉中肠出血性大肠杆菌O157:H7及其毒素的简单过滤技术。
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本文引用的文献

1
Identification of a clone of Escherichia coli O103:H2 as a potential agent of hemolytic-uremic syndrome in France.在法国，鉴定出一株大肠杆菌O103:H2克隆株为溶血尿毒综合征的潜在致病原。
J Clin Microbiol. 1993 Feb;31(2):296-301. doi: 10.1128/jcm.31.2.296-301.1993.
2
Identification of Escherichia coli serotype O157:H7 by DNA probe specific for an allele of uid A gene.通过针对uid A基因一个等位基因的DNA探针鉴定大肠杆菌O157:H7血清型。
Mol Cell Probes. 1993 Apr;7(2):151-4. doi: 10.1006/mcpr.1993.1021.
3
Examination of raw beef products for the presence of Vero cytotoxin producing Escherichia coli, particularly those of serogroup O157.检测生牛肉制品中是否存在产Vero细胞毒素的大肠杆菌，尤其是血清型O157的菌株。
J Appl Bacteriol. 1993 Nov;75(5):420-6. doi: 10.1111/j.1365-2672.1993.tb02797.x.
4
Virulence of an Escherichia coli O157:H7 sorbitol-positive mutant.一株大肠杆菌O157:H7山梨醇阳性突变株的毒力
Appl Environ Microbiol. 1993 Dec;59(12):4245-52. doi: 10.1128/aem.59.12.4245-4252.1993.
5
Shiga-like toxin-producing Escherichia coli in Seattle children: a prospective study.西雅图儿童中产志贺毒素大肠杆菌的前瞻性研究。
Gastroenterology. 1993 Dec;105(6):1724-31. doi: 10.1016/0016-5085(93)91069-t.
6
Genetic analysis of uidA expression in enterohaemorrhagic Escherichia coli serotype O157:H7.肠出血性大肠杆菌O157:H7中uidA基因表达的遗传分析。
Microbiology (Reading). 1994 Aug;140 ( Pt 8):2101-7. doi: 10.1099/13500872-140-8-2101.
7
Isolation of a Citrobacter freundii strain which carries the Escherichia coli O157 antigen.携带大肠杆菌O157抗原的弗氏柠檬酸杆菌菌株的分离
J Clin Microbiol. 1993 Mar;31(3):760-1. doi: 10.1128/jcm.31.3.760-761.1993.
8
Cloning and sequencing of the genes for Shiga toxin from Shigella dysenteriae type 1.1型痢疾志贺氏菌志贺毒素基因的克隆与测序
J Bacteriol. 1988 Mar;170(3):1116-22. doi: 10.1128/jb.170.3.1116-1122.1988.
9
Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.用于通过聚合酶链反应扩增不同志贺样毒素基因片段的单一引物对。
J Clin Microbiol. 1989 Dec;27(12):2751-7. doi: 10.1128/jcm.27.12.2751-2757.1989.
10
Infection by verocytotoxin-producing Escherichia coli.产志贺毒素大肠杆菌感染
Clin Microbiol Rev. 1989 Jan;2(1):15-38. doi: 10.1128/CMR.2.1.15.

通过错配扩增突变分析-多重聚合酶链反应同时鉴定大肠杆菌O157:H7血清型菌株及其志贺样毒素类型。

Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR.

作者信息

Cebula T A, Payne W L, Feng P

机构信息

Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D.C. 20204.

出版信息

J Clin Microbiol. 1995 Jan;33(1):248-50. doi: 10.1128/jcm.33.1.248-250.1995.

DOI:10.1128/jcm.33.1.248-250.1995
PMID:7535315
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC227922/
Abstract

Mismatch amplification mutation assay primers, specific for a unique base substitution in uidA of Escherichia coli O157:H7, was coupled with primers for the Shiga-like toxin I (SLT-I) and SLT-II genes in a multiplex PCR assay. Analysis of 108 bacteria showed that all Escherichia coli serotype O157:H7 strains were identified simultaneously with the SLT types encoded by these strains.

摘要

错配扩增突变分析引物，对大肠杆菌O157:H7的uidA中独特的碱基取代具有特异性，在多重PCR分析中与志贺样毒素I（SLT-I）和SLT-II基因的引物相结合。对108株细菌的分析表明，所有大肠杆菌O157:H7血清型菌株均与这些菌株编码的SLT类型同时被鉴定出来。