Galasso I, Lioi L, Lanave C, Bollini R, Sparvoli F
Istituto di Genetica Vegetale, CNR, via G. Amendola 165/A, 70126, Bari, Italy.
Theor Appl Genet. 2004 Apr;108(6):1098-102. doi: 10.1007/s00122-003-1520-9. Epub 2003 Nov 20.
Genes for lectin, a component of legume storage proteins, were identified and characterised in two lentil cultivars ( Lens culinaris ssp. culinaris) and six wild relatives. In each taxon no differences were found among the two or three lectin clones sequenced, while differences were observed among lectin genes isolated from the different taxa. All of the clones analysed contained an insert of 828 bp and showed a high similarity with the nucleotide sequence of Pisum sativum seed lectin, PSL1. The deduced amino acid lectin sequences in all taxa were 275 amino acids long, and their multiple alignment showed that most of the variation among them occurred in regions which are not important for metal- and sugar-binding. The data from Southern blot analysis indicated the presence of only one lectin gene in all Lens taxa except L. tomentosus. Phylogenetic analyses carried out on the lectin sequences showed the existence of two main clusters and clearly indicated that L. nigricans falls outside the two groups.
在两个小扁豆品种（Lens culinaris ssp. culinaris）和六个野生近缘种中鉴定并表征了豆类储存蛋白成分凝集素的基因。在每个分类群中，对两到三个测序的凝集素克隆未发现差异，而从不同分类群分离的凝集素基因之间存在差异。所有分析的克隆都包含一个828 bp的插入片段，并且与豌豆种子凝集素PSL1的核苷酸序列高度相似。所有分类群中推导的氨基酸凝集素序列长度为275个氨基酸，它们的多重比对表明，它们之间的大多数变异发生在对金属和糖结合不重要的区域。Southern印迹分析的数据表明，除绒毛小扁豆外，所有小扁豆分类群中仅存在一个凝集素基因。对凝集素序列进行的系统发育分析显示存在两个主要类群，并清楚地表明黑小扁豆不属于这两个类群。
