Wasfi Ibrahim A, Al-Awadhi Ahmed Hassan, Al-Hatali Zainat Naser, Al-Rayami Fatima Juma, Al Katheeri Nawal Abdulla
Camelracing Laboratory, Forensic Science Laboratory, P.O. Box 253, Abu Dhabi, United Arab Emirates.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jan 25;799(2):331-6. doi: 10.1016/j.jchromb.2003.11.003.
A sensitive and specific method using static headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in biological fluids using n-propanol as internal standard. Gas chromatography was performed in isothermal mode with a GC run time of 2.6 min. The quantification was performed using scan mode abstracting a quantitative ion and a qualifier ion for ethanol and for the internal standard. The method was linear (r(2), 0.999, in the concentration range of 5-200 mg/dl), specific (no interference from methanol acetaldehyde, acetone or from endogenous materials), sensitive (limit of quantification and limit of detection of 0.2 and 0.02 mg/dl, respectively) and robust (less than 5% inter- and intra-assay coefficient of variation). A slightly modified method was also developed for the quantification of five commonly abused inhalants (dichloromethane, ethyl acetate, benzene, toluene and xylene) in blood. The method used a gradient GC program with a run time of 8 min. The quantification was performed using scan mode and integrating the area under the peak using trichloroethane as an internal standard. Without optimization, the method was linear (from 5 to 100 mg/l) and sensitive.
已开发出一种灵敏且特异的方法，该方法采用静态顶空气相色谱-质谱联用（GC/MS），以正丙醇作为内标物，用于定量测定生物体液中的乙醇。气相色谱采用等温模式，GC运行时间为2.6分钟。定量分析通过扫描模式进行，提取乙醇和内标物的定量离子和定性离子。该方法具有线性（在5 - 200 mg/dl浓度范围内，r(2)为0.999）、特异（不受甲醇、乙醛、丙酮或内源性物质干扰）、灵敏（定量限和检测限分别为0.2和0.02 mg/dl）以及稳健（批间和批内变异系数小于5%）的特点。还开发了一种略有修改的方法，用于定量测定血液中五种常见滥用吸入剂（二氯甲烷、乙酸乙酯、苯、甲苯和二甲苯）。该方法采用梯度GC程序，运行时间为8分钟。定量分析通过扫描模式进行，并以内标物三氯乙烷积分峰面积。未经优化，该方法具有线性（5至100 mg/l）且灵敏。
