Jones A W, Mårdh G, Anggård E
Pharmacol Biochem Behav. 1983;18 Suppl 1:267-72. doi: 10.1016/0091-3057(83)90184-3.
We describe methods for the determination of endogenous ethanol in biological specimens from healthy abstaining subjects. The analytical methods were headspace gas chromatography (GC) for plasma samples and gas chromatography-mass spectometry (GC/MS) with deuterium labelled species 2H3-ethanol and 2H5-ethanol as internal standards for breath analysis. Ethanol in rebreathed air was about 10% higher than in directly analysed end-expired alveolar air. Known volumes of rebreathed air were passed through a liquid-N2 freeze trap and the volatile constituents of breath were concentrated prior to analysis by GC or GC/MS. Besides endogenous ethanol, peaks were seen on the chromatograms for methanol, acetone and acetaldehyde as well as several as yet unidentified substances. The endogenous alcohols ethanol and methanol were confirmed from their mass chromatograms and the GC/MS profile also indicated the presence of endogenous propan-1-ol. The concentration of endogenous ethanol in plasma showed wide inter-subject variations ranging from below detection limits to 1.6 micrograms/ml (34.8 mumol/l) and with mean +/- SD of 0.39 +/- 0.45 micrograms/ml (8.5 +/- 9.8 mumol/l). We aim to characterise further the role of endogenous ethanol with the main focus on dynamic aspects such as the rate of formation and turnover.
我们描述了测定健康戒酒受试者生物样本中内源性乙醇的方法。分析方法为：血浆样本采用顶空气相色谱法（GC），呼气分析采用气相色谱 - 质谱联用仪（GC/MS），以氘标记的2H3 - 乙醇和2H5 - 乙醇作为内标。再呼吸空气中的乙醇比直接分析呼出的终末肺泡气中的乙醇高约10%。已知体积的再呼吸空气通过液氮冷冻捕集器，呼气中的挥发性成分在通过GC或GC/MS分析之前进行浓缩。除了内源性乙醇外，色谱图上还出现了甲醇、丙酮和乙醛的峰以及几种尚未鉴定的物质。从其质量色谱图中确认了内源性醇类乙醇和甲醇，GC/MS图谱也表明存在内源性丙醇。血浆中内源性乙醇的浓度在受试者之间存在很大差异，范围从低于检测限到1.6微克/毫升（34.8微摩尔/升），平均±标准差为0.39±0.45微克/毫升（8.5±9.8微摩尔/升）。我们旨在进一步阐明内源性乙醇的作用，主要关注其动态方面，如生成速率和周转情况。
