Schreiner G F, Cotran R S, Pardo V, Unanue E R
J Exp Med. 1978 Feb 1;147(2):369-84. doi: 10.1084/jem.147.2.369.
An accelerated form of nephrotoxic serum nephritis in the rat was examined. The experimental model consisted of preimmunization of the rat with rabbit IgG 5 days before injection of subnephrotoxic doses of rabbit anti-rat kidney serum. The immunized rats developed proteinuria during the first 24 h, increasing by 48-96 h. The early 24-h proteinuria correlated with a neutrophilic infiltration of glomeruli and with deposition of rat Ig and C. The 48- to 96-h proteinuria was associated with a glomerular infiltration by mononuclear cells and proliferation of intrinsic glomerular cells. Many of the mononuclear cells were morphologically identical to monocytes and macrophages. [3H]thymidine labeling experiments indicated that the mononuclear cells originated from dividing precursors localized outside the kidney. Preimmunized rats given systemic irradiation (the kidney being protected by a shield) showed loss of the mononuclear cell infiltrate and absence of 48- to 96-h proteinuria. We conclude that mononuclear phagocytes can infiltrate the kidney in immunological glomerular disease and might contribute to the functional abnormalities.
对大鼠中加速形式的肾毒性血清肾炎进行了研究。实验模型包括在注射亚肾毒性剂量的兔抗大鼠肾血清前5天,用兔IgG对大鼠进行预免疫。免疫大鼠在最初24小时内出现蛋白尿,并在48 - 96小时增加。早期24小时蛋白尿与肾小球中性粒细胞浸润以及大鼠Ig和C的沉积相关。48至96小时的蛋白尿与单核细胞的肾小球浸润和固有肾小球细胞增殖有关。许多单核细胞在形态上与单核细胞和巨噬细胞相同。[3H]胸腺嘧啶核苷标记实验表明,单核细胞起源于位于肾脏外的分裂前体细胞。给予全身照射(肾脏用屏蔽物保护)的预免疫大鼠显示单核细胞浸润消失,且无48至96小时蛋白尿。我们得出结论,单核吞噬细胞可在免疫性肾小球疾病中浸润肾脏,并可能导致功能异常。