Bradfield J W, Cattell V
Lab Invest. 1977 May;36(5):481-6.
One-shot active immune complex glomerulonephritis was induced in rabbits by intravenous bovine serum albumin (250 mg. per kg.) and the mechanism of glomerular hypercellularity investigated. Most of the extra cells were mononuclear with few polymorphonuclear leukocytes. Fibrin was present in severe lesions. Glomerular mitoses were seen in normal kidneys but were more common in hypercellular glomeruli. This is direct evidence of local proliferation. Also, biopsies taken 1 hour after giving tritiated thymidine contained locally labeled cells. The mitotic rate and degree of local labeling both varied in proportion to the degree of hypercellularity. By electron microscopy both mesangial and endothelial cells were identified in mitosis. The majority of the mononuclear cells in the hypercellular glomeruli could not be identified by position or ultrastructure. Infiltration by nonglomerular cells was confirmed by the presence of macrophages, lymphocytes, and plasma cells.
通过静脉注射牛血清白蛋白（每千克250毫克）在兔中诱发一次性活性免疫复合物肾小球肾炎，并研究肾小球细胞增多的机制。大多数额外的细胞是单核细胞，仅有少量多形核白细胞。严重病变中存在纤维蛋白。正常肾脏中可见肾小球有丝分裂，但在细胞增多的肾小球中更常见。这是局部增殖的直接证据。此外，给予氚标记胸腺嘧啶核苷1小时后进行的活检含有局部标记的细胞。有丝分裂率和局部标记程度均与细胞增多程度成比例变化。通过电子显微镜观察，可见系膜细胞和内皮细胞处于有丝分裂状态。细胞增多的肾小球中的大多数单核细胞无法通过位置或超微结构鉴定。巨噬细胞、淋巴细胞和浆细胞的存在证实了非肾小球细胞的浸润。
