Nagai M, Ito T, Sugita S, Genno A, Takeuchi K, Ozawa T, Sakoda Y, Nishimori T, Takamura K, Akashi H
Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa, Japan.
Arch Virol. 2001;146(4):685-96. doi: 10.1007/s007050170139.
Genomic properties of 62 field isolates of bovine viral diarrhea virus (BVDV) collected from 1974 to 1999 in Japan were investigated. The 5' untranslated region (UTR) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and the 244 to 247 base nucleotide sequences were determined. Serological properties were also characterized by the cross-neutralization test using antisera against the representative strain of the classified genotype. Using phylogenetic tree analysis, BVDV 1 was subdivided into two major clusters, BVDV-1a (29 isolates) and BVDV-1b (27 isolates). In group 1a, 3 differed from the other viruses, and were classified in a branch assigned as 1a'. However, 4 isolates (So CP/75, 190 CP, 190 NCP and KS86-1-NCP) could not be assigned to group 1a or 1b. In comparison with the published sequence data, KS86-1-NCP, 190 CP and 190 NCP were similar to the Southern Africa isolates that have recently been proposed as BVDV 1c. The 5' UTR sequence of So CP/75 was unique among those of BVDV 1, suggesting that the isolate should be classified into a new genotype. Only 2 out of 62 isolates collected in 1989 and 1990 were identified as BVDV 2. The results of the cross-neutralization test strongly supported these data, suggesting a close correlation between the 5' UTR sequence and the antigenicity of BVDV.
对1974年至1999年期间在日本收集的62株牛病毒性腹泻病毒（BVDV）野毒株的基因组特性进行了研究。通过逆转录聚合酶链反应（RT-PCR）扩增5'非翻译区（UTR），并测定了244至247个碱基的核苷酸序列。还使用针对分类基因型代表性毒株的抗血清通过交叉中和试验对血清学特性进行了表征。通过系统发育树分析，BVDV 1被细分为两个主要簇，BVDV-1a（29株）和BVDV-1b（27株）。在1a组中，有3株与其他病毒不同，被归类到一个指定为1a'的分支中。然而，4株分离株（So CP/75、190 CP、190 NCP和KS86-1-NCP）无法归类到1a组或1b组。与已发表的序列数据相比，KS86-1-NCP、190 CP和190 NCP与最近被提议为BVDV 1c的南非分离株相似。So CP/75的5'UTR序列在BVDV 1的序列中是独特的，表明该分离株应归类为一个新的基因型。1989年和1990年收集的62株分离株中只有2株被鉴定为BVDV 2。交叉中和试验的结果有力地支持了这些数据，表明BVDV的5'UTR序列与抗原性之间存在密切相关性。
