Wang Youdong, Joh Keiichiro, Masuko Sadahiko, Yatsuki Hitomi, Soejima Hidenobu, Nabetani Akira, Beechey Colin V, Okinami Satoshi, Mukai Tsunehiro
Department of Biomolecular Sciences, Saga Medical School, Saga 849-8501, Japan.
Mol Cell Biol. 2004 Jan;24(1):270-9. doi: 10.1128/MCB.24.1.270-279.2004.
The mouse Murr1 gene contains an imprinted gene, U2af1-rs1, in its first intron. U2af1-rs1 shows paternal allele-specific expression and is transcribed in the direction opposite to that of the Murr1 gene. In contrast to a previous report of biallelic expression of Murr1 in neonatal mice, we have found that the maternal allele is expressed predominantly in the adult brain and also preferentially in other adult tissues. This maternal-predominant expression is not observed in embryonic and neonatal brains. In situ hybridization experiments that used the adult brain indicated that Murr1 gene was maternally expressed in neuronal cells in all regions of the brain. We analyzed the developmental change in the expression levels of both Murr1 and U2af1-rs1 in the brain and liver, and we propose that the maternal-predominant expression of Murr1 results from transcriptional interference of the gene by U2af1-rs1 through the Murr1 promoter region.
小鼠Murr1基因的第一个内含子中包含一个印记基因U2af1-rs1。U2af1-rs1呈现父本等位基因特异性表达，并且其转录方向与Murr1基因相反。与之前关于新生小鼠中Murr1双等位基因表达的报道不同，我们发现母本等位基因在成年大脑中占主导表达，在其他成年组织中也优先表达。在胚胎和新生大脑中未观察到这种母本占主导的表达。使用成年大脑进行的原位杂交实验表明，Murr1基因在大脑所有区域的神经元细胞中呈母本表达。我们分析了大脑和肝脏中Murr1和U2af1-rs1表达水平的发育变化，并提出Murr1的母本占主导表达是由于U2af1-rs1通过Murr1启动子区域对该基因进行转录干扰所致。
