Fries A S, Svendsen O
Lab Anim. 1978 Jan;12(1):1-4. doi: 10.1258/002367778780953297.
An outbreak of an epidemic disease occurred in a specified-pathogen-free (SPF) breeding colony of rats. The clinical signs and the post-mortem findings were characteristic for Tyzzer's disease. The causative agent, Bacillus piliformis, was demonstrated microscopically in ileum, liver and myocardium, and transmitted to mice where its pathogenicity appeared to be similar to that of another strain isolated from mice. B. piliformis from spontaneously-infected rats was demonstrated by indirect immunofluorescence technique. By means of the same technique it was found that the fluorescence antibody titre obtained of the individual sera from spontaneously-infected mice, rats and rabbits was the same, whether the antigen employed was organisms isolated from rats or mice. By testing sera from healthy rats in 3 different colonies by use of immunofluorescence technique, antibodies were found in several sera.
在一个无特定病原体（SPF）的大鼠繁殖群体中发生了一场流行病爆发。临床症状和尸检结果具有泰泽氏病的特征。致病因子，毛样芽孢杆菌，在回肠、肝脏和心肌中通过显微镜检查得以证实，并传播给小鼠，其致病性似乎与从小鼠分离出的另一菌株相似。通过间接免疫荧光技术证实了来自自发感染大鼠的毛样芽孢杆菌。通过相同技术发现，无论使用的抗原是从大鼠还是小鼠分离的生物体，从自发感染的小鼠、大鼠和兔子的个体血清中获得的荧光抗体滴度是相同的。通过使用免疫荧光技术检测来自3个不同群体的健康大鼠的血清，在几份血清中发现了抗体。
