Yaksh T L, Rudy T A
School of Pharmacy, University of Wisconsin, Madison, WI 54706, USA.
Physiol Behav. 1976 Dec;17(6):1031-6. doi: 10.1016/0031-9384(76)90029-9.
To administer drugs into the spinal subarachnoid space of unanesthetized and intact rats and rabbits, a procedure is described whereby a polyethylene catheter (PE-10) may be inserted through a puncture of the atlanto-occipital membrane and secured to the skull. Calibration experiments carried out with bromophenol blue dye, 3H-naloxone and 14C-urea revealed first, that there was little rostro-caudal diffusion of the injectate along the spinal axis and secondly, that even for compounds such as naloxone which can rapidly permeate neural tissues, the levels which do appear in the brain are small following the spinal subarachnoid administration of the drug. Control injections, administered either acutely or repeatedly over a prolonged period of time, had no detectable effect on the animal's behavior. These observations, as well as the lack of pathology in the spinal cords of rats having such catheters for periods of up to 4 months suggests that the implant is well tolerated.
为了将药物注入未麻醉且完整的大鼠和兔子的脊髓蛛网膜下腔，描述了一种方法，即可以通过寰枕膜穿刺插入聚乙烯导管（PE - 10）并固定在颅骨上。用溴酚蓝染料、³H - 纳洛酮和¹⁴C - 尿素进行的校准实验首先表明，注射剂沿脊髓轴的头尾扩散很小，其次，即使对于像纳洛酮这样能够快速渗透神经组织的化合物，在脊髓蛛网膜下腔给药后，脑中出现的水平也很低。急性或长时间重复进行的对照注射对动物行为没有可检测到的影响。这些观察结果，以及长达4个月植入此类导管的大鼠脊髓中没有病理学变化，表明该植入物耐受性良好。
