Sekerina O A, Chemerilova V I
Irkutsk State University, Sukhe-Bator ul. 5, Irkutsk, 664003 Russia.
Mikrobiologiia. 2003 Sep-Oct;72(5):689-94.
The process of dissociation into variants differing in colony morphology occurring in batch cloned cultures of two Bacillus thuringiensis strains belonging to different subspecies was studied at optimal and elevated temperatures. An increase in the cultivation temperature to 40 degrees C resulted in an increase in the fraction of R variants to 100% after 72 h of cultivation of either of the strains. This increase was not due to the selection of forms with greater resistance to elevated temperature. The level of resistance to elevated temperature was determined by the strain genotype and did not correlate with morphological characteristics of the colonies.
在最佳温度和高温条件下，研究了属于不同亚种的两种苏云金芽孢杆菌菌株的分批克隆培养物中，解离为菌落形态不同的变体的过程。将培养温度提高到40摄氏度，导致任一菌株培养72小时后，R变体的比例增加到100%。这种增加并非由于选择了对高温具有更高抗性的形式。对高温的抗性水平由菌株基因型决定，与菌落的形态特征无关。
