Blokhina T P, Sakharova Z V, Ignatenko Iu N, Rabotnova I L, Rautenshteĭn Ia I
Mikrobiologiia. 1984 May-Jun;53(3):427-31.
When a lysogenic culture of Bacillus thuringiensis subsp. galleriae 69-6 was grown under the batch conditions, 93-99% of cells in the population produced R-form colonies and ca. 1% yielded S-form colonies. The amount of spore-forming cells was 99% in R-variants and 8% in S-variants. The quantity of S-variants rose abruptly to 99% when the culture was grown under the chemostat conditions. The number of S-variants increased with the rate and the duration of growth. The process was influenced by growth-limiting factors. Temperate phage variants capable of host culture lysis on solid media (i.e. h-mutants) were not found under the conditions of batch cultivation. However, such phage particles (h-mutants) appeared under the conditions of chemostat. The titre of these phage particles reached 10(8), 10(7) and 10(4) particles per 1 ml at limitation with yeast extract, glucose and phosphorus, respectively. Under the conditions of chemostat, the particles behaved as temperate ones and their growth was not found. Irrespective of the limitation, the phage titre did not correlate with the ratio of R and S-forms in the population. When the growth was limited with phosphorus, the quantity of S-forms increased abruptly while the spontaneous induction of the phage was inhibited. The quantity of cells capable of spore formation decreased in the cultures isolated from the chemostat and grown on MPA: 69-80% of the cells in R-forms and merely 8% in S-forms.
当苏云金芽孢杆菌蜡螟亚种69-6的溶原培养物在分批培养条件下生长时，群体中93%-99%的细胞形成R型菌落，约1%产生S型菌落。R型变体中形成芽孢的细胞数量为99%，S型变体中为8%。当培养物在恒化器条件下生长时，S型变体的数量突然升至99%。S型变体的数量随着生长速率和持续时间而增加。该过程受生长限制因素的影响。在分批培养条件下未发现能够在固体培养基上裂解宿主培养物的温和噬菌体变体（即h-突变体）。然而，在恒化器条件下出现了此类噬菌体颗粒（h-突变体）。在分别以酵母提取物、葡萄糖和磷为限制因子时，这些噬菌体颗粒的滴度分别达到每1 ml 10⁸、10⁷和10⁴个颗粒。在恒化器条件下，这些颗粒表现为温和噬菌体，未发现其生长。无论限制因子如何，噬菌体滴度与群体中R型和S型的比例均无相关性。当生长受磷限制时，S型的数量突然增加，同时噬菌体的自发诱导受到抑制。从恒化器中分离并在MPA上生长的培养物中，能够形成芽孢的细胞数量减少：R型细胞中69%-80%，S型细胞中仅8%。
