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[苏云金芽孢杆菌蜡螟亚种连续培养过程中形成的变体的生物学特性]

[Biological characteristics of the variants of Bacillus thuringiensis subsp. galleriae formed during continuous culture].

作者信息

Blokhina T P, Rautenshteĭn Ia I, Sakharova Z V, Rabotnova I L, Zaboĭskaia R A

出版信息

Mikrobiologiia. 1985 Jul-Aug;54(4):683-4.

PMID:4058330
Abstract

Bacillus thuringiensis subsp. galleriae S. variants formed during continuous cultivation differ from the parent culture in certain properties. In contrast to the parent R form, their growth in the chemostat does not yield virulent mutants which can cause their lysis on solid media. The chemostat S forms are resistant against virulent phage mutants produced when the R variants are grown under the conditions of continuous cultivation and against a virulent phage obtained from the parent culture 69-6 under the action of vancomycin. The R forms are sensitive to these phages. When the S forms are grown under the chemostat conditions, they do not revert to the R forms. The R and S forms do not differ noticeably in the character of their growth, formation of spores and crystals, and biological activity.

摘要

在连续培养过程中形成的苏云金芽孢杆菌蜡螟亚种S型变体在某些特性上与亲代培养物不同。与亲代R型相比,它们在恒化器中的生长不会产生能在固体培养基上导致其裂解的毒性突变体。恒化器S型对R型变体在连续培养条件下生长时产生的毒性噬菌体突变体以及在万古霉素作用下从亲代培养物69-6获得的毒性噬菌体具有抗性。R型对这些噬菌体敏感。当S型在恒化器条件下生长时,它们不会回复到R型。R型和S型在生长特性、孢子和晶体形成以及生物活性方面没有明显差异。

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