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[苏云金芽孢杆菌芽孢形成菌株和无芽孢形成菌株的比较特征]

[Comparative characteristics of spore-forming and asporogenic strains of Bacillus thuringiensis].

作者信息

Azizbekian R R, Belykh R A, Netyksa E M, Pogosbekova M R

出版信息

Genetika. 1978 Mar;14(3):510-8.

PMID:640382
Abstract

Comparative characteristics of sporogenous and asporogenous Bacillus thuringiensis strains is carried out. Asporogenous strains are found to differ from wild type strains in a number of criteria, including colony morphology, character of growth on rich and poor media and UV-sensitivity. Sporogenous strains form R colonies, they are more stable and more rare produce variants forming S colonies. S colonies are typical for asporogenous mutants, and under the cultivation in unfavourable conditions (elevated temperature, a shift of pH, a change of an incubation regime) asporogenous strains dissociate with a high frequency into R form. Initial strains, which are multiple auxotrophs, under certain conditions can form "prototrophic" revertants which are unstable when incubated on rich media. Suppressor mutation is supposed to be a possible mechanism of the origination of "prototrophs".

摘要

对产孢型和不产孢型苏云金芽孢杆菌菌株的比较特征进行了研究。发现不产孢型菌株在许多标准上与野生型菌株不同,包括菌落形态、在丰富和贫瘠培养基上的生长特性以及对紫外线的敏感性。产孢型菌株形成R菌落,它们更稳定,很少产生形成S菌落的变体。S菌落是不产孢型突变体的典型特征,在不利条件(高温、pH值变化、培养条件改变)下培养时,不产孢型菌株会高频解离为R型。最初的菌株是多重营养缺陷型,在某些条件下可以形成“原养型”回复突变体,这些回复突变体在丰富培养基上培养时不稳定。推测抑制突变可能是“原养型”产生的一种机制。

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