Serra M, Morini M C, Scotlandi K, Fisher L W, Zini N, Colombo M P, Campanacci M, Maraldi N M, Olivari S, Baldini N
Laboratorio di Ricerca Oncologica, Istituti Ortopedici Rizzoli, Bologna, Italy.
Hum Pathol. 1992 Dec;23(12):1326-31. doi: 10.1016/0046-8177(92)90050-d.
Osteonectin (ON), a 32,000-kd glycoprotein involved in the early steps of mineralization of skeletal tissue, is a recognized differentiation marker of normal osteogenic cells. The expression of ON was evaluated in vitro and in tissue sections by the polyclonal antibody bON II. In different cell cultures immunocytochemistry and molecular biology displayed a nonspecific reaction for the antibody, which showed itself to be useless for the in vitro identification of cells of the osteoblastic lineage. The diagnostic use of bON II antibody was investigated by immunohistochemistry on a series of osteogenic and nonosteogenic bone tumors. A strongly positive stain of the entire neoplastic component of osteosarcoma and osteoblastoma and a weaker stain of the mononuclear component of giant cell tumor and chondroblastoma were observed. On the other hand, stains for chondrosarcoma, Ewing's sarcoma, fibrosarcoma, malignant fibrous histiocytoma, and brown tumor from hyperparathyroidism were entirely negative. Our results indicate that ON may be helpful in the histologic diagnosis of bone tumors, particularly in differentiating small cell osteosarcoma from other small round cell tumors.
骨连接素（ON）是一种分子量为32,000道尔顿的糖蛋白，参与骨骼组织矿化的早期步骤，是正常成骨细胞公认的分化标志物。通过多克隆抗体bON II在体外和组织切片中评估ON的表达。在不同的细胞培养物中，免疫细胞化学和分子生物学显示该抗体存在非特异性反应，这表明其对于体外鉴定成骨细胞系细胞毫无用处。通过对一系列成骨性和非成骨性骨肿瘤进行免疫组织化学研究，探讨了bON II抗体的诊断用途。观察到骨肉瘤和骨母细胞瘤的整个肿瘤成分呈强阳性染色，而巨细胞瘤和软骨母细胞瘤的单核成分呈弱阳性染色。另一方面，软骨肉瘤、尤因肉瘤、纤维肉瘤、恶性纤维组织细胞瘤以及甲状旁腺功能亢进引起的棕色瘤的染色均为阴性。我们的结果表明，骨连接素可能有助于骨肿瘤的组织学诊断，特别是在鉴别小细胞骨肉瘤与其他小圆形细胞肿瘤方面。
