Deng Xiao Dong, Wang Meng, Liu Si Hang, Xu Dian Long, Fei Xiao Wen
Key Laboratory of Tropical Transnational Medicine of Ministry of Education, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, China.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science & Key Laboratory of Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Haikou, China.
Front Plant Sci. 2025 Mar 17;16:1527439. doi: 10.3389/fpls.2025.1527439. eCollection 2025.
SKP1 (S-phase kinase-associated protein 1) is a key member of the SCF (SKP1-Cullin1-F-box) E3 ligase complex. The SCF complex is involved in regulating various levels of plant physiology, including regulation of cellular signaling and response to abiotic stresses. While the function of SKP1 in plants is well known, its function in algae remains poorly understood. In this study, we investigated the role of the gene using RNAi interference and overexpression approaches. Subcellular localization of SKP1 was performed by transient expression in onion epidermal cells. For abiotic stress assays, the growth of RNAi and overexpression recombinant strains was examined under conditions of high osmolality (sorbitol), high salinity (NaCl) and high temperature (37°C). Our results showed that silencing significantly reduced oil accumulation by 38%, whereas expressing led to a 37% increase in oil content, suggesting that plays a crucial role in regulating oil synthesis and may influence lipid accumulation by regulating photosynthetic carbon flux partitioning. Subcellular localization analysis revealed that was predominantly localized within the nucleus. Furthermore, our results showed that SKP1 responds to abiotic stresses. Under sorbol and NaCl stress conditions, RNAi interference strains exhibited better growth than controls; however, their growth was comparatively impaired under 37°C stress compared to controls. On the other hand, overexpression strains showed weaker growth under sorbol and NaCl stress but were more tolerant to 37°C heat stress. These results illustrate the functional diversity of SKP1 in . This study provides an important complement for lipid metabolism and abiotic stress regulation in microalgae.
SKP1（S期激酶相关蛋白1）是SCF（SKP1- Cul1- F盒）E3连接酶复合体的关键成员。SCF复合体参与调节植物生理的各个层面，包括细胞信号调节和对非生物胁迫的响应。虽然SKP1在植物中的功能已为人熟知，但其在藻类中的功能仍知之甚少。在本研究中，我们使用RNA干扰和过表达方法研究了该基因的作用。通过在洋葱表皮细胞中瞬时表达来进行SKP1的亚细胞定位。对于非生物胁迫测定，在高渗透压（山梨醇）、高盐度（氯化钠）和高温（37°C）条件下检测RNA干扰和过表达重组菌株的生长情况。我们的结果表明，基因沉默显著降低了38%的油脂积累，而过表达则导致油脂含量增加37%，这表明该基因在调节油脂合成中起关键作用，并且可能通过调节光合碳通量分配来影响脂质积累。亚细胞定位分析显示，该基因主要定位于细胞核内。此外，我们的结果表明SKP1对非生物胁迫有响应。在山梨醇和氯化钠胁迫条件下，RNA干扰菌株的生长比对照更好；然而，与对照相比，它们在37°C胁迫下的生长受到相对损害。另一方面，过表达菌株在山梨醇和氯化钠胁迫下生长较弱，但对37°C热胁迫更耐受。这些结果说明了SKP1在中的功能多样性。本研究为微藻脂质代谢和非生物胁迫调节提供了重要补充。
