College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Int J Mol Sci. 2023 Nov 16;24(22):16414. doi: 10.3390/ijms242216414.
Ubiquitination participates in plant hormone signaling and stress response to adversity. SKP1-Like, a core component of the SCF (Skp1-Cullin-F-box) complex, is the final step in catalyzing the ubiquitin-mediated protein degradation pathway. However, the gene family has not been well characterized in response to apple abiotic stresses and hormonal treatments. This study revealed that 17 gene family members with the conserved domain of SKP1 were identified in apples and were unevenly distributed on eight chromosomes. The genes located on chromosomes 1, 10, and 15 were highly homologous. The genes were divided into three subfamilies according to the evolutionary affinities of monocotyledons and dicotyledons. members of the same group or subfamily show some similarity in gene structure and conserved motifs. The predicted results of protein interactions showed that members of the MdSKP1-like family have strong interactions with members of the F-Box family of proteins. A selection pressure analysis showed that genes were in purifying selection. A chip data analysis showed that and were higher in flowers, whereas was higher in fruits. The upstream -elements of genes contained a variety of elements related to light regulation, drought, low temperature, and many hormone response elements, etc. Meanwhile, qRT-PCR also confirmed that the gene is indeed involved in the response of the apple to hormonal and abiotic stress treatments. This research provides evidence for regulating gene expression in response to hormonal and abiotic stresses to improve apple stress resistance.
泛素化参与植物激素信号转导和逆境应激反应。SKP1 样蛋白（SKP1-Like）是 SCF（Skp1-Cullin-F-box）复合物的核心组成部分，是催化泛素介导的蛋白质降解途径的最后一步。然而，该基因家族在应对苹果非生物胁迫和激素处理方面的特征尚未得到很好的描述。本研究在苹果中鉴定到了 17 个具有 SKP1 保守结构域的基因家族成员，并不均匀分布在 8 条染色体上。位于染色体 1、10 和 15 上的基因高度同源。根据单子叶植物和双子叶植物的进化亲缘关系，将基因分为三个亚家族。同一组或亚家族的成员在基因结构和保守基序上具有一定的相似性。蛋白质相互作用的预测结果表明，MdSKP1 样家族的成员与 F-Box 家族的成员具有很强的相互作用。选择压力分析表明，基因处于纯化选择状态。芯片数据分析表明，和在花中表达较高，而在果实中表达较高。基因上游启动子元件含有多种与光调控、干旱、低温和多种激素应答元件等相关的元件。同时，qRT-PCR 也证实该基因确实参与了苹果对激素和非生物胁迫处理的响应。本研究为调控基因表达以应对激素和非生物胁迫，提高苹果的抗逆性提供了证据。
