Liao Hui-Fen, Chen Yu-Ywan, Liu Jun-Jen, Hsu Ming-Ling, Shieh Hui-Ju, Liao Hung-Jen, Shieh Chwen-Jen, Shiao Ming-Shi, Chen Yu-Jen
Department of Medical Research and Radiation Oncology, Mackay Memorial Hospital, Taipei 104, Taiwan.
J Agric Food Chem. 2003 Dec 31;51(27):7907-12. doi: 10.1021/jf034729d.
Caffeic acid phenethyl ester (CAPE) derived from honeybee propolis has been used as a folk medicine and has several proven biological activities. The present study investigated the effect of CAPE on angiogenesis, tumor invasion, and metastasis. A cytotoxicity assay of CAPE in CT26 colon adenocarcinoma cells showed a dose-dependent decrease in cell viability but no significant influence on the growth of human umbilical vein epithelial cells (HUVEC). A low concentration of CAPE (1.5 microg/mL) inhibited 52.7% of capillary-like tube formation in HUVEC culture on Matrigel. CAPE (6 microg/mL)-treated CT26 cells showed not only inhibited cell invasion by 47.8% but also decreased expression of matrix metalloproteinase (MMP)-2 and -9. Vascular endothelial growth factor (VEGF) production from CT26 cells was also inhibited by treatment with CAPE (6 microg/mL). Intraperitoneal injection of CAPE (10 mg/kg/day) in BALB/c mice reduced the pulmonary metastatic capacity of CT26 cells accompanied with a decreased plasma VEGF level. CAPE treatment also prolonged the survival of mice implanted with CT26 cells. These results indicate that CAPE has potential as an antimetastatic agent.
源自蜜蜂蜂胶的咖啡酸苯乙酯（CAPE）一直被用作民间药物，并且具有多种已被证实的生物活性。本研究调查了CAPE对血管生成、肿瘤侵袭和转移的影响。CAPE对CT26结肠腺癌细胞的细胞毒性试验显示，细胞活力呈剂量依赖性下降，但对人脐静脉上皮细胞（HUVEC）的生长没有显著影响。低浓度的CAPE（1.5微克/毫升）抑制了基质胶上HUVEC培养中52.7%的毛细血管样管形成。用CAPE（6微克/毫升）处理的CT26细胞不仅显示细胞侵袭受到47.8%的抑制，而且基质金属蛋白酶（MMP）-2和-9的表达也降低。用CAPE（6微克/毫升）处理也抑制了CT26细胞中血管内皮生长因子（VEGF）的产生。在BALB/c小鼠中腹腔注射CAPE（10毫克/千克/天）降低了CT26细胞的肺转移能力，同时血浆VEGF水平降低。CAPE治疗还延长了植入CT26细胞的小鼠的生存期。这些结果表明，CAPE具有作为抗转移剂的潜力。
