Gribbon Philip, Sewing Andreas
Lead Discovery Technologies, Pfizer Global Research and Development, Ramsgate Road, Sandwich, UK CT13 9NJ.
Drug Discov Today. 2003 Nov 15;8(22):1035-43. doi: 10.1016/s1359-6446(03)02895-2.
Fluorescence-based detection technologies are frequently applied in biological testing, due to their unique advantages in setting up homogeneous, sensitive assays in miniaturized formats. However, the wide application of these readouts has highlighted challenges in reagent design and problems with interference from biological reagents and compounds. Here, we summarize the current application of fluorescence-based detection methodologies, focusing on the problems faced by assay developers and on solutions to reduce false positive and negative results in fluorescence-based HTS.
基于荧光的检测技术因其在以小型化形式建立均相、灵敏检测方面的独特优势，而经常应用于生物检测中。然而，这些读数的广泛应用凸显了试剂设计方面的挑战以及生物试剂和化合物干扰带来的问题。在此，我们总结了基于荧光的检测方法的当前应用，重点关注检测方法开发者所面临的问题以及减少基于荧光的高通量筛选中假阳性和假阴性结果的解决方案。
