Eggeling Christian, Brand Leif, Ullmann Dirk, Jäger Stefan
Evotec OAI / Evotec Technologies, Schnackenburgallee 114, D-22525 Hamburg, Germany.
Drug Discov Today. 2003 Jul 15;8(14):632-41. doi: 10.1016/s1359-6446(03)02752-1.
Homogeneous fluorescence methods are providing an important tool for HTS technologies. A wide range of different techniques have been established on the market, with read-outs ranging from total fluorescence intensity to statistical analysis of fluorescence fluctuations for biochemical assays or fluorescence imaging techniques for cellular systems. Each method has its own advantages and limitations, which have to be accounted for when designing a specific assay. Here, recently developed fluorescence techniques and some of their applications, with a particular focus on sensitivity, are summarized and their principles are presented.
均相荧光方法为高通量筛选技术提供了一种重要工具。市场上已建立了各种各样不同的技术，其读出方式从生化分析的总荧光强度到荧光波动的统计分析，再到细胞系统的荧光成像技术。每种方法都有其自身的优点和局限性，在设计特定分析方法时必须加以考虑。在此，总结了最近开发的荧光技术及其一些应用，特别关注灵敏度，并介绍了它们的原理。
