Gorinstein Shela, Caspi Abraham, Zemser Marina, Libman Imanuel, Goshev Ivan, Trakhtenberg Simon
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University - Hadassah Medical School, P.O.B. 12065, Jerusalem 91120, Israel.
J Nutr Biochem. 2003 Dec;14(12):710-6. doi: 10.1016/j.jnutbio.2003.09.002.
MODERATE BEER CONSUMPTION (MBC) IS CARDIOPROTECTIVE: it positively influences plasma lipid levels and plasma antioxidant activity in beer-consuming individuals. The connection between MBC and blood coagulation is not clearly defined. Forty-two volunteers were equally divided into experimental (EG) and control (CG) groups following coronary bypass surgery. For 30 consecutive days, only patients of the EG consumed 330 mL of beer per day (about 20 g of alcohol). A comprehensive clinical investigation of 42 patients was done. Blood samples were collected before and after the investigation for a wide range of laboratory tests. The plasma fibrinogen was denatured with 8 M urea and intrinsic fluorescence (IF), hydrophobicity and differential scanning calorimetry (DSC) were used to reveal possible qualitative changes. After 30 days of moderate beer consumption, positive changes in the plasma lipid levels, plasma anticoagulant and plasma antioxidant activities were registered in patients of the EG group. In 17 out of 21 patients of the same group, differences in plasma circulating fibrinogen's (PCF), secondary and tertiary structures were found. The stability of fibrinogen, expressed in thermodynamic parameters, has shown that the loosening of the structure takes place under ethanol and urea denaturation. Also fluorescence stability of PCF was decreased. No changes in the lipid levels, anticoagulant and antioxidant activity or changes in PCF were detected in patients of CG. In conclusion, for the first time after a short term of moderate beer consumption some qualitative changes in the plasma circulating fibrinogen were detected: differences in the emission peak response, fluorescence intensity and all thermodynamic data. Together, with the decrease in the PCF concentration it may lead to an elevation of the blood anticoagulant activity.
适度饮用啤酒（MBC）对心脏有保护作用：它对饮用啤酒者的血脂水平和血浆抗氧化活性有积极影响。MBC与血液凝固之间的联系尚未明确界定。42名志愿者在冠状动脉搭桥手术后被平均分为实验组（EG）和对照组（CG）。连续30天，只有EG组的患者每天饮用330毫升啤酒（约20克酒精）。对42名患者进行了全面的临床调查。在调查前后采集血样进行广泛的实验室检测。用8M尿素使血浆纤维蛋白原变性，并利用内在荧光（IF）、疏水性和差示扫描量热法（DSC）揭示可能的定性变化。适度饮用啤酒30天后，EG组患者的血脂水平、血浆抗凝和血浆抗氧化活性出现了积极变化。在同一组的21名患者中，有17名患者的血浆循环纤维蛋白原（PCF）的二级和三级结构存在差异。以热力学参数表示的纤维蛋白原稳定性表明，在乙醇和尿素变性条件下，结构发生了松散。PCF的荧光稳定性也降低了。CG组患者的血脂水平、抗凝和抗氧化活性未检测到变化，PCF也未发生变化。总之，在短期适度饮用啤酒后，首次检测到血浆循环纤维蛋白原出现了一些定性变化：发射峰响应、荧光强度和所有热力学数据存在差异。再加上PCF浓度的降低，可能会导致血液抗凝活性升高。
