Braun T, Frank H, Dods R, Sepsenwol S
Biochim Biophys Acta. 1977 Mar 15;481(1):227-35. doi: 10.1016/0005-2744(77)90155-3.
In subcellular fractions prepared from homogenate of adult rat testis adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) activity was found in the particulate, primarily 600 X g for 10 min, fractions, as well as in the cytosol. The properties of the adenylate cyclase in the cytosol differs substantially from the adenylate cyclase system associated with the 600 X g for 10 min particulate fraction. The cytosol enzyme, in contrast to the particulate adenylate cyclase, was found to be fluoride- and gonadotropin hormone-insensitive. The cytosol adenylate cyclase appears to be located in the germ cell while the particulate enzyme system in the non-germ cell component of the seminiferous tubules, The cytosol adenylate cyclase was found to be distinct also from the guanylate cyclase present in the rat testis cytosol. The adenylate cyclase appears to be located in the germ cell component while the guanylate cyclase, in the non-germ cell tubular component. Furthermore, it was found that the cytosol guanylate cyclase develops at an earlier stage of spermatogenesis, and precedes the development of the cytosol adenylate cyclase.
在从成年大鼠睾丸匀浆制备的亚细胞组分中，腺苷酸环化酶（ATP 焦磷酸裂解酶（环化），EC 4.6.1.1）活性在颗粒组分（主要是经 600×g 离心 10 分钟的组分）以及胞质溶胶中均被发现。胞质溶胶中腺苷酸环化酶的性质与和经 600×g 离心 10 分钟的颗粒组分相关的腺苷酸环化酶系统有很大不同。与颗粒性腺苷酸环化酶相反，发现胞质溶胶酶对氟化物和促性腺激素不敏感。胞质溶胶腺苷酸环化酶似乎位于生殖细胞中，而颗粒性酶系统存在于生精小管的非生殖细胞成分中。还发现胞质溶胶腺苷酸环化酶也与大鼠睾丸胞质溶胶中存在的鸟苷酸环化酶不同。腺苷酸环化酶似乎位于生殖细胞成分中，而鸟苷酸环化酶位于非生殖细胞的小管成分中。此外，发现胞质溶胶鸟苷酸环化酶在精子发生的较早阶段发育，并先于胞质溶胶腺苷酸环化酶的发育。
