Ziegler A, Natsuaki T, Mayo M A, Jolly C A, Murant A F
Scottish Crop Research Institute, Invergowrie, Dundee, U.K.
J Gen Virol. 1992 Dec;73 ( Pt 12):3213-8. doi: 10.1099/0022-1317-73-12-3213.
Raspberry bushy dwarf virus (RBDV) has isometric, 33 nm diameter particles and a bipartite RNA genome. Sequencing of the larger component (RNA-1) showed that it consists of 5449 nucleotides and contains one large open reading frame encoding a putative translation product with a calculated M(r) of 190,000. Comparisons of this polypeptide with non-structural proteins of other plant viruses revealed significant homologies with those of alfalfa mosaic virus (AlMV), brome mosaic virus (BMV), cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV). Thus RBDV belongs to the supergroup of 'Sindbis-like' plant viruses. The translation product of RBDV RNA-1 contains motifs characteristic of proteins with polymerase, methyltransferase and helicase activities, suggesting that this protein is involved in the replication of the viral RNA. Thus in RBDV, as in TMV, all three functional domains are combined in the single protein, whereas in AlMV, BMV and CMV these domains are distributed over the proteins encoded by RNA-1 and RNA-2. These findings support the idea that RBDV should be placed in a distinct virus genus for which the name idaeovirus has been proposed.
悬钩子矮化病毒（RBDV）具有直径33纳米的等轴粒体颗粒和双分体RNA基因组。对较大组分（RNA - 1）的测序表明，它由5449个核苷酸组成，包含一个大的开放阅读框，编码一个推定的翻译产物，计算的分子量为190,000。将该多肽与其他植物病毒的非结构蛋白进行比较，发现与苜蓿花叶病毒（AlMV）、雀麦花叶病毒（BMV）、黄瓜花叶病毒（CMV）和烟草花叶病毒（TMV）的非结构蛋白有显著同源性。因此，RBDV属于“辛德比斯样”植物病毒超群。RBDV RNA - 1的翻译产物包含具有聚合酶、甲基转移酶和解旋酶活性的蛋白质的特征基序，表明该蛋白参与病毒RNA的复制。因此，在RBDV中，如同在TMV中一样，所有三个功能域都组合在单一蛋白中，而在AlMV、BMV和CMV中，这些域分布在RNA - 1和RNA - 2编码的蛋白质上。这些发现支持将RBDV置于一个独特病毒属的观点，已有人提议将该属命名为鸢尾病毒属。
