Nucleotide sequence of raspberry bushy dwarf virus RNA-3.

A nucleotide sequence is reported for RNA-3, the smallest of the three major RNA species found in particles of raspberry bushy dwaft virus (RBDV). The sequence of 946 nucleotides contains a single large open reading frame which encodes an Mr 30,509 polypeptide. In vitro translation of RNA-3 yielded an Mr 30,000 product that reacted specifically with antiserum to RBDV particles and we conclude that the amino acid sequence deduced from the sequence of RNA-3 is that of the RBDV coat protein, or an immediate precursor of it. No affinities were detected by comparing the nucleotide sequence of RNA-3 with the sequences of other plant viruses.