Natsuaki T, Mayo M A, Jolly C A, Murant A F
Scottish Crop Research Institute, Invergowrie, Dundee U.K.
J Gen Virol. 1991 Sep;72 ( Pt 9):2183-9. doi: 10.1099/0022-1317-72-9-2183.
Northern blot analysis with cDNA probes to RNA-3 (1 kb) of raspberry bushy dwarf virus (RBDV) revealed extensive sequence homology with RBDV RNA-2 (2.2 kb). Nucleotide sequencing showed that RNA-2 contains two large open reading frames (ORFs), of 1074 (5' ORF) and 822 (3' ORF) bases. The 3' ORF is virtually identical in sequence to RNA-3, which encodes the Mr 30509 (30K) coat protein. The 5' ORF encodes an Mr 38860 (39K) protein which slightly resembles the 32K protein encoded by RNA-3 of alfalfa mosaic virus (AlMV). RBDV RNA-2 resembles AlMV RNA-3 in being bicistronic and encoding a coat protein at the 3' end. Comparing RNA-1 and RNA-2 of RBDV, only the 18 3'-terminal nucleotides are identical in sequence but the 3'-terminal 71 nucleotides of each RNA species have the potential to form similar stem-loop structures.
用树莓矮化病毒（RBDV）RNA - 3（1 kb）的cDNA探针进行的Northern印迹分析显示，其与RBDV RNA - 2（2.2 kb）具有广泛的序列同源性。核苷酸测序表明，RNA - 2包含两个大的开放阅读框（ORF），分别为1074个碱基（5' ORF）和822个碱基（3' ORF）。3' ORF在序列上与RNA - 3几乎相同，RNA - 3编码分子量为30509（30K）的衣壳蛋白。5' ORF编码分子量为38860（39K）的蛋白质，该蛋白质与苜蓿花叶病毒（AlMV）RNA - 3编码的32K蛋白质略有相似。RBDV RNA - 2在具有双顺反子结构并在3'端编码衣壳蛋白方面类似于AlMV RNA - 3。比较RBDV的RNA - 1和RNA - 2，只有18个3'末端核苷酸序列相同，但每个RNA种类的3'末端71个核苷酸都有可能形成相似的茎环结构。
