Saber Sara Mohamed, Alduweesh Nouf Bader, Abd El-Rahman Heba Ali, Omar Amel Ramadan
Department of Histopathology, National Organization for Drug Control and Research, (NODCAR), Egypt.
Department of Zoology, Faculty of Science, Kuwait University, Kuwait.
Saudi J Biol Sci. 2021 Apr;28(4):2558-2565. doi: 10.1016/j.sjbs.2021.01.060. Epub 2021 Feb 11.
Filgrastim is a recombinant protein used in treatment neutropenia caused by myelosuppressive medications for patients with non-myeloid cancer. However, its effect in male fertility is not clear. So, the current work aims to clarify the effect of Filgrastim on the reproductive state in Wistar rats. Eighteen (18) male Wistar rats were divided into three groups (6/each). Group (I) where the rats were injected with 0.5 ml/kg/day of distilled water and served as Control Group. The Group (II) animals received intraperitoneal injection of therapeutic dose of 30.83 mcg/kg/day of Filgrastim for one week. The Group (III) rats received the same dose by the same route of Filgrastim for two weeks. Sera of blood samples were processed for serum follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone (TS). Semen analysis and resazurine reduction test (RRT) were performed. Assaying for malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) was done. The testes were retrieved for histopathological and immunohistochemical studies for caspase-3 detection. Our results revealed that filgrastim affects sperm morphology, significantly decreased the RRT and the reproductive hormones level, elevated the oxidative stress status and induced several histopathological changes in testes with an increased in immunoexpression of caspase-3 in testes tissues. The results of this work demonstrated that Filgrastim may had a deleterious effect on male fertility.
非格司亭是一种重组蛋白，用于治疗非髓系癌症患者因骨髓抑制药物引起的中性粒细胞减少症。然而，其对男性生育能力的影响尚不清楚。因此，目前的研究旨在阐明非格司亭对Wistar大鼠生殖状态的影响。将18只雄性Wistar大鼠分为三组（每组6只）。第一组大鼠每天注射0.5 ml/kg的蒸馏水，作为对照组。第二组动物腹腔注射治疗剂量的非格司亭，剂量为30.83 mcg/kg/天，持续一周。第三组大鼠通过相同途径接受相同剂量的非格司亭，持续两周。对血样血清进行处理，检测血清卵泡刺激素（FSH）、黄体生成素（LH）、睾酮（TS）。进行精液分析和刃天青还原试验（RRT）。检测丙二醛（MDA）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）。取出睾丸进行组织病理学和免疫组织化学研究，以检测caspase-3。我们的结果显示，非格司亭影响精子形态，显著降低RRT和生殖激素水平，提高氧化应激状态，并在睾丸中诱导了几种组织病理学变化，睾丸组织中caspase-3的免疫表达增加。这项研究的结果表明，非格司亭可能对男性生育能力有有害影响。
