Soltani Malihe, Moghimian Maryam, Abtahi-Eivari Seyed Hossein, Shoorei Hamed, Khaki Arash, Shokoohi Majid
Department of Basic Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.
Department of Anatomical Sciences, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Int J Fertil Steril. 2018 Jun;12(3):242-248. doi: 10.22074/ijfs.2018.5324. Epub 2018 Jun 20.
There is some evidence indicating that Matricaria chamomile (MC) had protective effects on ischemiareperfusion. In the present study, a rat model was used to investigate the effect of hydroalcoholic extract of MC on torsion/detorsion-induced testis tissue damage.
In this experimental study, 28 male Wistar rats were randomly divided into 4 groups as follows: G1, Sham operated; G2, testicular torsion/detorsion (T/D); G3, rats with testicular torsion/detorsion that received 300 mg/kg of MC extracts 30 minutes before detorsion (T/DMC); and G4, healthy rats that received 300 mg/kg of MC extracts (MC). Also, the reperfusion period was 24 hours. After blood sampling, the oxidative stress marker [e.g. superoxide dismutase (SOD) levels], blood levels of testosterone, and anti-oxidant enzyme levels [e.g. glutathione peroxidase (GPx)] were assessed by ELISA methods. Serum activity of malondialdehyde (MDA) was evaluated by spectrophotometry. Another assessment was carried out by histomorphometry, 24-hour post-procedure. The histological parameters investigated by Johnson's scores (JS), also the seminiferous tubule diameter (STD) and the height of the germinal epithelium (HE) measured using the linear eyepiece grids using light microscopy.
Histological features significantly differed between sham and the other groups. The levels of SOD, GPx, and testosterone hormone were significantly decreased in T/D group as compared to sham group, while these parameters increased in T/DMC group as compared to T/D group. During ischemia, the MDA levels increased; however, treatment with MC extract decreased the MDA levels in G3 and G4 groups.
Results of the present study demonstrated that MC can protect the testis tissue against torsion/detorsioninduced damages by suppressing superoxide production.
有证据表明母菊花（MC）对缺血再灌注具有保护作用。在本研究中，使用大鼠模型来研究MC水醇提取物对扭转/去扭转诱导的睾丸组织损伤的影响。
在本实验研究中，28只雄性Wistar大鼠随机分为4组，如下：G1，假手术组；G2，睾丸扭转/去扭转组（T/D）；G3，睾丸扭转/去扭转大鼠，在去扭转前30分钟接受300mg/kg的MC提取物（T/DMC）；G4，接受300mg/kg MC提取物的健康大鼠（MC）。此外，再灌注期为24小时。采血后，通过ELISA方法评估氧化应激标志物[如超氧化物歧化酶（SOD）水平]、睾酮血水平和抗氧化酶水平[如谷胱甘肽过氧化物酶（GPx）]。通过分光光度法评估丙二醛（MDA）的血清活性。在手术后24小时通过组织形态计量学进行另一项评估。通过约翰逊评分（JS）研究组织学参数，还使用光学显微镜通过线性目镜网格测量生精小管直径（STD）和生精上皮高度（HE）。
假手术组与其他组之间的组织学特征有显著差异。与假手术组相比，T/D组的SOD、GPx和睾酮激素水平显著降低，而与T/D组相比，T/DMC组的这些参数有所增加。在缺血期间，MDA水平升高；然而，用MC提取物治疗可降低G3和G4组的MDA水平。
本研究结果表明，MC可通过抑制超氧化物产生来保护睾丸组织免受扭转/去扭转诱导的损伤。
