病理状态下胰腺腺泡细胞中伴侣蛋白60和胰腺酶的改变。
Alteration of chaperonin60 and pancreatic enzyme in pancreatic acinar cell under pathological condition.
作者信息
Li Yong-Yu, Bendayan Moise
机构信息
Department of Pathophysiology, Medical College of Tongji University, Shanghai 200092, China.
出版信息
World J Gastroenterol. 2005 Dec 14;11(46):7359-63. doi: 10.3748/wjg.v11.i46.7359.
AIM
To investigate the changes of chaperonin60 (Cpn60) and pancreatic enzymes in pancreatic acinar cells, and to explore their roles in the development of experimental diabetes and acute pancreatitis (AP).
METHODS
Two different pathological models were replicated in Sprague-Dawley rats: streptozotocin-induced diabetes and sodium deoxycholate-induced AP. The contents of Cpn60 and pancreatic enzymes in different compartments of the acinar cells were measured by quantitative immunocytochemistry.
RESULTS
The levels of Cpn60 significantly increased in diabetes, but decreased in AP, especially in the zymogen granules of the pancreatic acinar cells. The elevation of Cpn60 was accompanied with the increased levels of pancreatic lipase and chymotrypsinogen in diabetes. However, a decreased Cpn60 level was accompanied by high levels of lipase and chymotrypsinogen in AP. The amylase level was markedly reduced in both the pathological conditions.
CONCLUSION
The equilibrium between Cpn60 and pancreatic enzymes in the acinar cells breaks in AP, and Cpn60 content decreases, suggesting an insufficient chaperone capacity. This may promote the aggregation and autoactivation of the premature enzymes in the pancreatic acinar cells and play roles in the development of AP.
目的
研究伴侣蛋白60（Cpn60）和胰腺酶在胰腺腺泡细胞中的变化，并探讨它们在实验性糖尿病和急性胰腺炎（AP）发展中的作用。
方法
在Sprague-Dawley大鼠中复制两种不同的病理模型：链脲佐菌素诱导的糖尿病和脱氧胆酸钠诱导的AP。通过定量免疫细胞化学测量腺泡细胞不同区室中Cpn60和胰腺酶的含量。
结果
糖尿病时Cpn60水平显著升高，但AP时降低，尤其是在胰腺腺泡细胞的酶原颗粒中。糖尿病时Cpn60升高伴随着胰腺脂肪酶和胰凝乳蛋白酶原水平的增加。然而，AP时Cpn60水平降低伴随着脂肪酶和胰凝乳蛋白酶原水平升高。两种病理状态下淀粉酶水平均显著降低。
结论
AP时腺泡细胞中Cpn60与胰腺酶之间的平衡被打破，Cpn60含量降低，提示伴侣功能不足。这可能促进胰腺腺泡细胞中过早酶的聚集和自激活，并在AP的发展中起作用。
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