内毒素诱导性葡萄膜炎兔房水中丙二醛和抗氧化酶水平

Malondialdehyde and antioxidant enzyme levels in the aqueous humor of rabbits in endotoxin-induced uveitis.

作者信息

Satici A, Guzey M, Gurler B, Vural H, Gurkan T

机构信息

Department of Ophthalmology, School of Medicine, Harran University, Sanliurfa, Turkey.

出版信息

Eur J Ophthalmol. 2003 Nov-Dec;13(9-10):779-83. doi: 10.1177/1120672103013009-1008.

DOI:10.1177/1120672103013009-1008

PMID:14700099

Abstract

PURPOSE

To investigate the role of oxidative stress in endotoxin-induced uveitis.

METHODS

Lipopolysaccharide was injected intravitreally into the right eyes of rabbits. Sterile saline was injected intravitreally into the left eyes as a control. Inflammation was assessed according to clinical score, aqueous humor cell count, and protein levels. Malondialdehyde, superoxide dismutase, glutathione peroxidase, catalase, and nitrite levels were measured in the aqueous humor.

RESULTS

The clinical grade (p < 0.01), inflammatory cell count (p < 0.001), and protein content (p < 0.001) were significantly higher in the aqueous humor of eyes with uveitis than in that of controls. Malondialdehyde (p < 0.01) and nitrite (p < 0.001) levels in the aqueous humor of eyes with uveitis were significantly higher than in the control group. Superoxide dismutase (p < 0.001), glutathione peroxidase (p < 0.001), and catalase (p < 0.001) levels were significantly lower in the aqueous humor of eyes with uveitis than in that of the controls.

CONCLUSIONS

Oxygen free radicals may be implicated as a mediator of inflammation in endotoxin-induced uveitis. The increase in free radicals in the aqueous humor may play a role in the pathogenesis of endotoxin-induced uveitis.

摘要

目的

研究氧化应激在内毒素诱导性葡萄膜炎中的作用。

方法

将脂多糖玻璃体内注射入兔右眼。将无菌生理盐水玻璃体内注射入左眼作为对照。根据临床评分、房水细胞计数和蛋白水平评估炎症。测量房水中丙二醛、超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶和亚硝酸盐水平。

结果

葡萄膜炎眼房水的临床分级（p < 0.01）、炎症细胞计数（p < 0.001）和蛋白含量（p < 0.001）显著高于对照组。葡萄膜炎眼房水中丙二醛（p < 0.01）和亚硝酸盐（p < 0.001）水平显著高于对照组。葡萄膜炎眼房水中超氧化物歧化酶（p < 0.001）、谷胱甘肽过氧化物酶（p < 0.001）和过氧化氢酶（p < 0.001）水平显著低于对照组。