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植物硫肽激素刺激日本柳杉的体细胞胚胎发生。

Phytosulfokine stimulates somatic embryogenesis in Cryptomeria japonica.

作者信息

Igasaki Tomohiro, Akashi Noriko, Ujino-Ihara Tokuko, Matsubayashi Yoshikatsu, Sakagami Youji, Shinohara Kenji

机构信息

Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute (FFPRI), Tsukuba, 305-8687 Japan.

出版信息

Plant Cell Physiol. 2003 Dec;44(12):1412-6. doi: 10.1093/pcp/pcg161.

DOI:10.1093/pcp/pcg161
PMID:14701937
Abstract

Phytosulfokine (PSK), which has been identified as a plant growth factor, had a dramatic stimulatory effect on the formation of somatic embryos of sugi (Cryptomeria japonica) in the presence of polyethylene glycol. The resultant somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots, and most of the seedlings grew normally. A cDNA clone for the precursor to the PSK peptide of C. japonica was identified in an expressed sequence tags database. Our results support the existence of a PSK signaling pathway in C. japonica.

摘要

植物磺肽素(PSK)已被鉴定为一种植物生长因子,在聚乙二醇存在的情况下,它对日本柳杉(Cryptomeria japonica)体细胞胚的形成具有显著的刺激作用。由此产生的体细胞胚会随着子叶、下胚轴和根的同步萌发而发芽,并且大多数幼苗生长正常。在一个表达序列标签数据库中鉴定出了日本柳杉PSK肽前体的cDNA克隆。我们的结果支持日本柳杉中存在PSK信号通路。

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