Alto Barry W, Lounibos L Philip, Juliano Steven A
University of Florida, Florida Medical Entomology Laboratory, 200 9th Street SE, Vero Beach, FL 32962, USA.
J Am Mosq Control Assoc. 2003 Dec;19(4):347-52.
In order to quantify age-dependent acquisition of a bloodmeal, we compared bloodfeeding patterns of Aedes aegypti and Aedes albopictus starting from the time of first responsiveness to a bloodmeal up to 15 days postemergence. In separate experiments, cohorts of Ae. aegypti and Ae. albopictus of known age were offered their first bloodmeal from a silicon-membrane system or a restrained chicken. For cohorts ranging from 3 to 15 days old, the proportions feeding were significantly affected by species, age, feeding protocol, and the age x feeding protocol interaction. For both feeding protocols, a higher proportion of Ae. aegypti than Ae. albopictus, on average, consumed blood. Regressions of proportion feeding versus age indicated significantly positive slopes for Ae. albopictus and Ae. aegypti on the membrane system, and no significant relationship was observed for either species on the restrained chicken. Additionally, slopes for Aedes, as a group, fed on the membrane system were significantly different from those fed on the living host. For both Aedes species fed on the restrained chicken and for Ae. aegypti fed on the membrane system, the proportions feeding exhibited periodic patterns, with peaks approximately 2 days apart, suggesting possible control by endogenous rhythms.
为了量化按蚊吸食血餐的年龄依赖性，我们比较了埃及伊蚊和白纹伊蚊从首次对血餐有反应开始到羽化后15天的吸血模式。在单独的实验中，将已知年龄的埃及伊蚊和白纹伊蚊群体从硅膜系统或受限鸡身上获取它们的第一顿血餐。对于3至15日龄的群体，吸血比例受到物种、年龄、取食方案以及年龄×取食方案相互作用的显著影响。对于两种取食方案，平均而言，埃及伊蚊吸血的比例高于白纹伊蚊。吸血比例与年龄的回归分析表明，白纹伊蚊和埃及伊蚊在膜系统上的斜率显著为正，而在受限鸡身上两种物种均未观察到显著关系。此外，作为一个群体，在膜系统上取食的伊蚊的斜率与在活体宿主上取食的伊蚊的斜率显著不同。对于在受限鸡身上取食的两种伊蚊物种以及在膜系统上取食的埃及伊蚊，吸血比例呈现出周期性模式，峰值间隔约为2天，这表明可能受内源性节律控制。
