Department of Pathology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0609, USA.
Centro de Entomologia, Fundação de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil.
Viruses. 2022 Dec 23;15(1):45. doi: 10.3390/v15010045.
(Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F generation eggs for the first time. A total of 62.8% (1562/2486) F generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79/92) female F mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of past the F generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle.
（Dyar，1921）是主要的新热带丛林型黄热病病毒的媒介，它极难在人工环境下饲养。自 20 世纪 40 年代以来，它从未被培育到 F 代以上，而且几乎没有用这种物种进行过任何实验性传播研究。在此，我们描述了分期孵化、人工血液喂养和强制交配技术，这些技术使我们首次能够生产少量 F 代卵。在≤10 个为期四天的竹叶浸提液浸泡周期中，F 代卵中有 62.8%（1562/2486）孵化，随后部分干燥。在通过强制交配交配的蚊子产下的 F 代卵中，孵化率降至 20.1%（190/944）。超过 85%（79/92）的 F 代雌性蚊子在人工血液喂养系统上进食。虽然我们无法将实验室种群维持在 F 代以上，但我们的方法为在实验室环境中用该物种进行实验性传播研究提供了基础，在登革热、寨卡和基孔肯雅热病毒高度流行传播的地区，这是一个关键能力，所有这些病毒都有可能溢出到丛林型循环中。
