Disease Vector Group, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, 230 54, Alnarp, Sweden.
Business Unit Biointeractions and Plant Health, Wageningen University and Research, AA, 6700, Wageningen, The Netherlands.
BMC Genomics. 2021 Jan 21;22(1):71. doi: 10.1186/s12864-020-07336-w.
In the light of dengue being the fastest growing transmissible disease, there is a dire need to identify the mechanisms regulating the behaviour of the main vector Aedes aegypti. Disease transmission requires the female mosquito to acquire the pathogen from a blood meal during one gonotrophic cycle, and to pass it on in the next, and the capacity of the vector to maintain the disease relies on a sustained mosquito population.
Using a comprehensive transcriptomic approach, we provide insight into the regulation of the odour-mediated host- and oviposition-seeking behaviours throughout the first gonotrophic cycle. We provide clear evidence that the age and state of the female affects antennal transcription differentially. Notably, the temporal- and state-dependent patterns of differential transcript abundance of chemosensory and neuromodulatory genes extends across families, and appears to be linked to concerted differential modulation by subsets of transcription factors.
By identifying these regulatory pathways, we provide a substrate for future studies targeting subsets of genes across disparate families involved in generating key vector behaviours, with the goal to develop novel vector control tools.
鉴于登革热是增长最快的传染性疾病，因此迫切需要确定调节主要病媒埃及伊蚊行为的机制。疾病传播需要雌性蚊子在一个配子生殖周期中从血液餐中获得病原体，并在下一个周期中传播它，而病媒维持疾病的能力依赖于持续的蚊子种群。
使用综合转录组学方法，我们深入了解了整个第一个配子生殖周期中气味介导的宿主和产卵寻求行为的调节。我们提供了明确的证据，表明雌蚊的年龄和状态会对触角转录产生不同的影响。值得注意的是，化学感觉和神经调质基因的差异转录丰度的时相和状态依赖性模式跨越了家族，并且似乎与转录因子亚群的协调差异调节有关。
通过鉴定这些调控途径，我们为未来的研究提供了一个基础，这些研究针对涉及产生关键病媒行为的不同家族中的亚组基因，目标是开发新的病媒控制工具。
