Sen Moon M, Adhikari Atin, Gupta-Bhattacharya Swati, Chanda Sunirmal
Division of Palynology and Environmental Biology, Department of Botany, Bose Institute, 9311 Acharya Prafulla Chandra Road, Kolkata (Calcutta)- 700 009, India.
J Environ Monit. 2003 Dec;5(6):959-62. doi: 10.1039/b302453a.
Grass pollens are well known among the health hazardous bioaerosols causing respiratory allergy. Being an important member of the grass family, the rice plants contribute a huge pollen load in agricultural fields during flowering. This results in a seasonal trigger of hay fever and respiratory allergy in the field workers and people living in the vicinity. Studies on the monitoring of airborne rice pollen and the intensity of the released allergen in agricultural fields are largely lacking. The aims of the present study were: (1) daily and hourly monitoring of airborne rice pollen in an agricultural field during the flowering period of plants in a winter crop season by using the Burkard 7-Day Volumetric Sampler and (2) the measurement of hourly airborne allergen intensity in the field in a peak rice pollen period by the double-antibody and chemiluminescence techniques to find out its relationship with the airborne rice pollen concentration. The monthly average concentration of rice pollen was 95 pollen m(-3) and the range of daily average pollen concentration was 0 to 386 pollen m(-3). A bimodal diurnal periodicity showed that the airborne rice pollen concentrations remained high at two different times of the day: between 08:00 h to 12:00 h and 14:00 h to 16:00 h. Deposition of airborne rice pollen allergen showed darker intensities on the immunostained tapes from the Burkard Sampler at the same two positions corresponding to higher pollen counts. These observations provided direct evidence of the allergenicity of airborne rice pollen in field conditions.
草花粉是众所周知的对健康有害的生物气溶胶，可引发呼吸道过敏。作为禾本科的重要成员，水稻植株在开花期会在农田中释放大量花粉。这导致田间工人和居住在附近的人季节性地引发花粉热和呼吸道过敏。目前在监测农田空气中水稻花粉及其释放的过敏原强度方面的研究非常缺乏。本研究的目的是：（1）在冬季作物季节植物开花期，使用伯卡德7天容量采样器对农田空气中的水稻花粉进行每日和每小时监测；（2）在水稻花粉高峰期，通过双抗体和化学发光技术测量田间空气中每小时的过敏原强度，以找出其与空气中水稻花粉浓度的关系。水稻花粉的月平均浓度为95粒花粉/立方米，日平均花粉浓度范围为0至386粒花粉/立方米。双峰昼夜周期性表明，空气中水稻花粉浓度在一天中的两个不同时间保持较高水平：08:00至12:00以及14:00至16:00。在与较高花粉计数相对应的相同两个位置，来自伯卡德采样器的免疫染色带上，空气中水稻花粉过敏原的沉积显示出更深的强度。这些观察结果提供了田间条件下空气中水稻花粉具有致敏性的直接证据。
