优化体外脱囊后分离的完整微小隐孢子虫卵囊对新生小鼠的感染性。
Neonatal-mouse infectivity of intact Cryptosporidium parvum oocysts isolated after optimized in vitro excystation.
作者信息
Hou L, Li X, Dunbar L, Moeller R, Palermo B, Atwill E R
机构信息
Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California-Davis, Tulare, California 93274, USA.
出版信息
Appl Environ Microbiol. 2004 Jan;70(1):642-6. doi: 10.1128/AEM.70.1.642-646.2004.
Abstract
We reexamined the finding of Neumann et al. that intact Cryptosporidium parvum oocysts obtained after in vitro excystation were infectious for neonatal CD-1 mice. We used both established excystation protocols and our own protocol that maximized excystation. Although intact oocysts isolated after any of three protocols were infectious for neonatal CD-1 mice, the infectivity of intact oocysts isolated with our optimized excystation protocol was significantly lower than the infectivity of intact oocysts isolated after established protocols or from fresh oocysts. Excystation should not be considered a valid measure of C. parvum viability, given that it is biologically implausible for oocysts to be nonviable and yet infectious.
摘要
我们重新审视了诺伊曼等人的研究结果，即在体外脱囊后获得的完整微小隐孢子虫卵囊对新生CD-1小鼠具有感染性。我们使用了既定的脱囊方案以及我们自己能使脱囊最大化的方案。尽管通过三种方案中的任何一种分离出的完整卵囊对新生CD-1小鼠都具有感染性，但用我们优化的脱囊方案分离出的完整卵囊的感染性明显低于通过既定方案或从新鲜卵囊中分离出的完整卵囊的感染性。鉴于卵囊无活力却具有感染性在生物学上是不合理的，因此脱囊不应被视为衡量微小隐孢子虫活力的有效指标。
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本文引用的文献
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