Smith Charles L
Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota 55415, USA.
J Urol. 2004 Feb;171(2 Pt 1):882-4. doi: 10.1097/01.ju.0000105250.62440.e3.
A role for the mucous lining of the urinary tract to prevent crystal adherence as well as the ability of glycosaminoglycans and other substances to restore anti-adherence after removal of the mucous lining was studied in the rat bladder.
Following catheterization of the rat bladder a dilute solution of acid was introduced, which has been shown to remove the mucous lining. Sialic acid, hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparin, pentosan polysulfate and urine were introduced into the bladder in an attempt to restore the anti-adherence properties removed by acid treatment. Radioactive calcium oxalate crystals were introduced into the bladder, followed by saline washes, and the crystals remaining were used as a measure of crystal adherence. Controls were mucous intact and acid treated bladders.
Hyaluronic acid, chondroitin-6-sulfate and chondroitin-4-sulfate did not influence adherence. Dermatan sulfate, heparin and pentosan polysulfate restored anti-adherence, while sialic acid and urine promoted adherence.
The mucous lining of the urinary tract serves as a defense against calcium oxalate crystal adherence. Dermatan sulfate decreases crystal adherence, whereas sialic acid promotes adherence. The balance between these 2 factors may have a role in stone formation.
在大鼠膀胱中研究泌尿道黏液层在防止晶体黏附方面的作用，以及糖胺聚糖和其他物质在去除黏液层后恢复抗黏附能力的情况。
对大鼠膀胱进行插管后，引入稀酸溶液，该溶液已被证明可去除黏液层。将唾液酸、透明质酸、硫酸软骨素、硫酸皮肤素、肝素、聚硫酸戊聚糖和尿液引入膀胱，试图恢复经酸处理后被去除的抗黏附特性。将放射性草酸钙晶体引入膀胱，随后用盐水冲洗，剩余的晶体用作晶体黏附的指标。对照组为黏液完整的膀胱和经酸处理的膀胱。
透明质酸、硫酸软骨素 - 6 - 硫酸盐和硫酸软骨素 - 4 - 硫酸盐不影响黏附。硫酸皮肤素、肝素和聚硫酸戊聚糖恢复了抗黏附能力，而唾液酸和尿液促进了黏附。
泌尿道的黏液层可作为防止草酸钙晶体黏附的一种防御机制。硫酸皮肤素可降低晶体黏附，而唾液酸则促进黏附。这两种因素之间的平衡可能在结石形成中起作用。
