Shnyreva A V, Belokon' Iu S, Belokon' M M
Department of Mycology and Algology, Lomonosov Moscow State University, Moscow, 119992 Russia.
Genetika. 2003 Nov;39(11):1461-9.
Analysis of commercial strains of two edible mushrooms, Pleurotus ostreatus and Agaricus bisporus, using PCR and isozyme electrophoresis techniques allowed us to differentiate groups of genetically similar and distant strains. Among the commercial strains of P. ostreatus, the level of genetic variation was higher suggesting a broader genetic basis employed in breeding of this mushroom. The cultivars and hybrids of, A. bisporus, showed a higher level of homology. The isozyme markers (nonspecific esterase, leucinaminopeptidase, and phosphoglucoisomerase) are recommended for identification of the commercial strains of edible mushrooms.
利用聚合酶链式反应（PCR）和同工酶电泳技术对两种食用菌平菇和双孢蘑菇的商业菌株进行分析，使我们能够区分基因相似和差异较大的菌株组。在平菇的商业菌株中，遗传变异水平较高，这表明该蘑菇育种采用了更广泛的遗传基础。双孢蘑菇的栽培品种和杂交种表现出较高的同源性。推荐使用同工酶标记（非特异性酯酶、亮氨酰氨基肽酶和磷酸葡萄糖异构酶）来鉴定食用菌的商业菌株。
